From Proteopedia
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| - | [[Image:1mx7.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1mx7| PDB=1mx7 | SCENE= }} | | {{STRUCTURE_1mx7| PDB=1mx7 | SCENE= }} |
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| - | '''Two homologous rat cellular retinol-binding proteins differ in local structure and flexibility'''
| + | ===Two homologous rat cellular retinol-binding proteins differ in local structure and flexibility=== |
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| - | ==Overview==
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| - | Cellular retinol-binding protein I (CRBP I) and cellular retinol-binding protein II (CRBP II) are closely homologous proteins that play distinct roles in the maintenance of vitamin A homeostasis. The solution structure and dynamics of CRBP I and CRBP II were compared by multidimensional NMR techniques. These studies indicated that differences in the mean backbone structures of CRBP I and CRBP II were localized primarily to the alphaII helix. Intraligand NOE cross-peaks were detected for the hydroxyl proton in the NOESY spectrum of CRBP I-bound retinol, but not for CRBP II-bound retinol, indicating that the conformational dynamics of retinol binding are different for these two proteins. As determined by Lipari-Szabo formalism, both the apo and holo forms of CRBP I and CRBP II are conformationally rigid on the pico- to nanosecond timescale. transverse relaxation optimized spectroscopy-Carr-Purcell-Meiboom-Gill -based 15N relaxation dispersion experiments at both 500 MHz and 600 MHz magnetic fields revealed that 84 and 62 residues for apo-CRBP I and II, respectively, showed detectable conformational exchange on a micro- to millisecond timescale, in contrast to three and seven residues for holo-CRBP I and II, respectively. Thus binding of retinol markedly reduced conformational flexibility in both CRBP I and CRBP II on the micro- to millisecond timescale. The 15N relaxation dispersion curves of apo-CRBP I and II were fit to a two-state conformational exchange model by a global iterative fitting process and by an individual (residue) fitting process. In the process of carrying out the global fit, more than half of the residue sites were eliminated. The individual chemical exchange rates k(ex), and chemical shift differences, Deltadelta, were increased in the putative portal region (alphaII helix and betaC-betaD turn) of apo-CRBP II compared to apo-CRBP I. These differences in conformational flexibility likely contribute to differences in how CRBP I and CRBP II interact with ligands, membranes and retinoid metabolizing enzymes.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12850148}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12850148 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12850148}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1MX7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MX7 OCA]. | + | 1MX7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MX7 OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Transport]] | | [[Category: Transport]] |
| | [[Category: Vitamin some]] | | [[Category: Vitamin some]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:49:46 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:09:16 2008'' |
Revision as of 16:09, 27 July 2008
Template:STRUCTURE 1mx7
Two homologous rat cellular retinol-binding proteins differ in local structure and flexibility
Template:ABSTRACT PUBMED 12850148
About this Structure
1MX7 is a Single protein structure of sequence from Rattus norvegicus. Full experimental information is available from OCA.
Reference
Two homologous rat cellular retinol-binding proteins differ in local conformational flexibility., Lu J, Cistola DP, Li E, J Mol Biol. 2003 Jul 18;330(4):799-812. PMID:12850148
Page seeded by OCA on Sun Jul 27 19:09:16 2008
