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1sfe

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{{STRUCTURE_1sfe| PDB=1sfe | SCENE= }}
{{STRUCTURE_1sfe| PDB=1sfe | SCENE= }}
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'''ADA O6-METHYLGUANINE-DNA METHYLTRANSFERASE FROM ESCHERICHIA COLI'''
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===ADA O6-METHYLGUANINE-DNA METHYLTRANSFERASE FROM ESCHERICHIA COLI===
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==Overview==
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The mutagenic and carcinogenic effects of simple alkylating agents are mainly due to methylation at the O6 position of guanine in DNA. O6-methylguanine directs the incorporation of either thymine or cytosine without blocking DNA replication, resulting in GC to AT transition mutations. In prokaryotic and eukaryotic cells antimutagenic repair is effected by direct reversal of this DNA damage. A suicidal methyltransferase repair protein removes the methyl group from DNA to one of its own cysteine residues. The resulting self-methylation of the active site cysteine renders the protein inactive. Here we report the X-ray structure of the 19 kDa C-terminal domain of the Escherichia coli ada gene product, the prototype of these suicidal methyltransferases. In the crystal structure the active site cysteine is buried. We propose a model for the significant conformational change that the protein must undergo in order to bind DNA and effect methyl transfer.
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(as it appears on PubMed at http://www.pubmed.gov), where 8156986 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8156986}}
==About this Structure==
==About this Structure==
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[[Category: Nucleic acid binding protein]]
[[Category: Nucleic acid binding protein]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:38:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:10:22 2008''

Revision as of 16:10, 27 July 2008

Image:1sfe.png

Template:STRUCTURE 1sfe

ADA O6-METHYLGUANINE-DNA METHYLTRANSFERASE FROM ESCHERICHIA COLI

Template:ABSTRACT PUBMED 8156986

About this Structure

1SFE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of a suicidal DNA repair protein: the Ada O6-methylguanine-DNA methyltransferase from E. coli., Moore MH, Gulbis JM, Dodson EJ, Demple B, Moody PC, EMBO J. 1994 Apr 1;13(7):1495-501. PMID:8156986

Page seeded by OCA on Sun Jul 27 19:10:22 2008

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