2azo

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{{STRUCTURE_2azo| PDB=2azo | SCENE= }}
{{STRUCTURE_2azo| PDB=2azo | SCENE= }}
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'''DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI'''
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===DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI===
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==Overview==
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MutS, MutL and MutH are the three essential proteins for initiation of methyl-directed DNA mismatch repair to correct mistakes made during DNA replication in Escherichia coli. MutH cleaves a newly synthesized and unmethylated daughter strand 5' to the sequence d(GATC) in a hemi-methylated duplex. Activation of MutH requires the recognition of a DNA mismatch by MutS and MutL. We have crystallized MutH in two space groups and solved the structures at 1.7 and 2.3 A resolution, respectively. The active site of MutH is located at an interface between two subdomains that pivot relative to one another, as revealed by comparison of the crystal structures, and this presumably regulates the nuclease activity. The relative motion of the two subdomains in MutH correlates with the position of a protruding C-terminal helix. This helix appears to act as a molecular lever through which MutS and MutL may communicate the detection of a DNA mismatch and activate MutH. With sequence homology to Sau3AI and structural similarity to PvuII endonuclease, MutH is clearly related to these enzymes by divergent evolution, and this suggests that type II restriction endonucleases evolved from a common ancestor.
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(as it appears on PubMed at http://www.pubmed.gov), where 9482749 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9482749}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Muth]]
[[Category: Muth]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:40:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:13:46 2008''

Revision as of 16:13, 27 July 2008

Image:2azo.png

Template:STRUCTURE 2azo

DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI

Template:ABSTRACT PUBMED 9482749

About this Structure

2AZO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases., Ban C, Yang W, EMBO J. 1998 Mar 2;17(5):1526-34. PMID:9482749

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