2gl9

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{{STRUCTURE_2gl9| PDB=2gl9 | SCENE= }}
{{STRUCTURE_2gl9| PDB=2gl9 | SCENE= }}
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'''Crystal Structure of Glycosylasparaginase-Substrate Complex'''
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===Crystal Structure of Glycosylasparaginase-Substrate Complex===
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==Overview==
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Glycosylasparaginase (GA) plays an important role in asparagine-linked glycoprotein degradation. A deficiency in the activity of human GA leads to a lysosomal storage disease named aspartylglycosaminuria. GA belongs to a superfamily of N-terminal nucleophile hydrolases that autoproteolytically generate their mature enzymes from inactive single chain protein precursors. The side-chain of the newly exposed N-terminal residue then acts as a nucleophile during substrate hydrolysis. By taking advantage of mutant enzyme of Flavobacterium meningosepticum GA with reduced enzymatic activity, we have obtained a crystallographic snapshot of a productive complex with its substrate (NAcGlc-Asn), at 2.0 A resolution. This complex structure provided us an excellent model for the Michaelis complex to examine the specific contacts critical for substrate binding and catalysis. Substrate binding induces a conformational change near the active site of GA. To initiate catalysis, the side-chain of the N-terminal Thr152 is polarized by the free alpha-amino group on the same residue, mediated by the side-chain hydroxyl group of Thr170. Cleavage of the amide bond is then accomplished by a nucleophilic attack at the carbonyl carbon of the amide linkage in the substrate, leading to the formation of an acyl-enzyme intermediate through a negatively charged tetrahedral transition state.
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(as it appears on PubMed at http://www.pubmed.gov), where 17157318 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17157318}}
==About this Structure==
==About this Structure==
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[[Category: Oxyanion hole]]
[[Category: Oxyanion hole]]
[[Category: Proton-relay network]]
[[Category: Proton-relay network]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:13:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:18:44 2008''

Revision as of 16:18, 27 July 2008

Image:2gl9.png

Template:STRUCTURE 2gl9

Crystal Structure of Glycosylasparaginase-Substrate Complex

Template:ABSTRACT PUBMED 17157318

About this Structure

2GL9 is a Protein complex structure of sequences from Elizabethkingia meningoseptica. Full crystallographic information is available from OCA.

Reference

Crystallographic snapshot of a productive glycosylasparaginase-substrate complex., Wang Y, Guo HC, J Mol Biol. 2007 Feb 9;366(1):82-92. Epub 2006 Sep 26. PMID:17157318

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