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| - | [[Image:2rds.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2rds| PDB=2rds | SCENE= }} | | {{STRUCTURE_2rds| PDB=2rds | SCENE= }} |
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| - | '''Crystal Structure of PtlH with Fe/oxalylglycine and ent-1-deoxypentalenic acid bound'''
| + | ===Crystal Structure of PtlH with Fe/oxalylglycine and ent-1-deoxypentalenic acid bound=== |
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| - | ==Overview==
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| - | The non-heme iron dioxygenase PtlH from the soil organism Streptomyces avermitilis is a member of the iron(II)/alpha-ketoglutarate-dependent dioxygenase superfamily and catalyzes an essential reaction in the biosynthesis of the sesquiterpenoid antibiotic pentalenolactone. To investigate the structural basis for substrate recognition and catalysis, we have determined the x-ray crystal structure of PtlH in several complexes with the cofactors iron, alpha-ketoglutarate, and the non-reactive enantiomer of the substrate, ent-1-deoxypentalenic acid, in four different crystal forms to up to 1.31 A resolution. The overall structure of PtlH forms a double-stranded barrel helix fold, and the cofactor-binding site for iron and alpha-ketoglutarate is similar to other double-stranded barrel helix fold enzymes. Additional secondary structure elements that contribute to the substrate-binding site in PtlH are not conserved in other double-stranded barrel helix fold enzymes. Binding of the substrate enantiomer induces a reorganization of the monoclinic crystal lattice leading to a disorder-order transition of a C-terminal alpha-helix. The newly formed helix blocks the major access to the active site and effectively traps the bound substrate. Kinetic analysis of wild type and site-directed mutant proteins confirms a critical function of two arginine residues in substrate binding, while simulated docking of the enzymatic reaction product reveals the likely orientation of bound substrate. | + | The line below this paragraph, {{ABSTRACT_PUBMED_17942405}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17942405 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17942405}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Double stranded barrel helix]] | | [[Category: Double stranded barrel helix]] |
| | [[Category: Oxidoreductase]] | | [[Category: Oxidoreductase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:42:23 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:32:48 2008'' |
Revision as of 16:32, 27 July 2008
Template:STRUCTURE 2rds
Crystal Structure of PtlH with Fe/oxalylglycine and ent-1-deoxypentalenic acid bound
Template:ABSTRACT PUBMED 17942405
About this Structure
2RDS is a Single protein structure of sequence from Streptomyces avermitilis. Full crystallographic information is available from OCA.
Reference
Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis., You Z, Omura S, Ikeda H, Cane DE, Jogl G, J Biol Chem. 2007 Dec 14;282(50):36552-60. Epub 2007 Oct 16. PMID:17942405
Page seeded by OCA on Sun Jul 27 19:32:48 2008
