1n9s

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{{STRUCTURE_1n9s| PDB=1n9s | SCENE= }}
{{STRUCTURE_1n9s| PDB=1n9s | SCENE= }}
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'''Crystal structure of yeast SmF in spacegroup P43212'''
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===Crystal structure of yeast SmF in spacegroup P43212===
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==Overview==
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Sm and Sm-like proteins are key components of small ribonucleoproteins involved in many RNA and DNA processing pathways. In eukaryotes, these complexes contain seven unique Sm or Sm-like (Lsm) proteins assembled as hetero-heptameric rings, whereas in Archaea and bacteria six or seven-membered rings are made from only a single polypeptide chain. Here we show that single Sm and Lsm proteins from yeast also have the capacity to assemble into homo-oligomeric rings. Formation of homo-oligomers by the spliceosomal small nuclear ribonucleoprotein components SmE and SmF preclude hetero-interactions vital to formation of functional small nuclear RNP complexes in vivo. To better understand these unusual complexes, we have determined the crystal structure of the homomeric assembly of the spliceosomal protein SmF. Like its archaeal/bacterial homologs, the SmF complex forms a homomeric ring but in an entirely novel arrangement whereby two heptameric rings form a co-axially stacked dimer via interactions mediated by the variable loops of the individual SmF protein chains. Furthermore, we demonstrate that the homomeric assemblies of yeast Sm and Lsm proteins are capable of binding not only to oligo(U) RNA but, in the case of SmF, also to oligo(dT) single-stranded DNA.
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{{ABSTRACT_PUBMED_12618433}}
==About this Structure==
==About this Structure==
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[[Category: Sm protein]]
[[Category: Sm protein]]
[[Category: Snrnp]]
[[Category: Snrnp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:16:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:35:55 2008''

Revision as of 16:35, 27 July 2008

Image:1n9s.png

Template:STRUCTURE 1n9s

Crystal structure of yeast SmF in spacegroup P43212

Template:ABSTRACT PUBMED 12618433

About this Structure

1N9S is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Homomeric ring assemblies of eukaryotic Sm proteins have affinity for both RNA and DNA. Crystal structure of an oligomeric complex of yeast SmF., Collins BM, Cubeddu L, Naidoo N, Harrop SJ, Kornfeld GD, Dawes IW, Curmi PM, Mabbutt BC, J Biol Chem. 2003 May 9;278(19):17291-8. Epub 2003 Mar 4. PMID:12618433

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