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1trh

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{{STRUCTURE_1trh| PDB=1trh | SCENE= }}
{{STRUCTURE_1trh| PDB=1trh | SCENE= }}
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'''TWO CONFORMATIONAL STATES OF CANDIDA RUGOSA LIPASE'''
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===TWO CONFORMATIONAL STATES OF CANDIDA RUGOSA LIPASE===
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==Overview==
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The structure of Candida rugosa lipase in a new crystal form has been determined and refined at 2.1 A resolution. The lipase molecule was found in an inactive conformation, with the active site shielded from the solvent by a part of the polypeptide chain-the flap. Comparison of this structure with the previously determined "open" form of this lipase, in which the active site is accessible to the solvent and presumably the substrate, shows that the transition between these 2 states requires only movement of the flap. The backbone NH groups forming the putative oxyanion hole do not change position during this rearrangement, indicating that this feature is preformed in the inactive state. The 2 lipase conformations probably correspond to states at opposite ends of the pathway of interfacial activation. Quantitative analysis indicates a large increase of the hydrophobic surface in the vicinity of the active site. The flap undergoes a flexible rearrangement during which some of its secondary structure refolds. The interactions of the flap with the rest of the protein change from mostly hydrophobic in the inactive form to largely hydrophilic in the "open" conformation. Although the flap movement cannot be described as a rigid body motion, it has very definite hinge points at Glu 66 and at Pro 92. The rearrangement is accompanied by a cis-trans isomerization of this proline, which likely increases the energy required for the transition between the 2 states, and may play a role in the stabilization of the active conformation at the water/lipid interface. Carbohydrate attached at Asn 351 also provides stabilization for the open conformation of the flap.
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(as it appears on PubMed at http://www.pubmed.gov), where 8142901 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8142901}}
==About this Structure==
==About this Structure==
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[[Category: Cygler, M.]]
[[Category: Cygler, M.]]
[[Category: Grochulski, P.]]
[[Category: Grochulski, P.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:17:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:43:25 2008''

Revision as of 16:43, 27 July 2008

Image:1trh.png

Template:STRUCTURE 1trh

TWO CONFORMATIONAL STATES OF CANDIDA RUGOSA LIPASE

Template:ABSTRACT PUBMED 8142901

About this Structure

1TRH is a Single protein structure of sequence from Candida rugosa. Full crystallographic information is available from OCA.

Reference

Two conformational states of Candida rugosa lipase., Grochulski P, Li Y, Schrag JD, Cygler M, Protein Sci. 1994 Jan;3(1):82-91. PMID:8142901

Page seeded by OCA on Sun Jul 27 19:43:25 2008

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