2yu9
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(New page: 200px<br /><applet load="2yu9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2yu9, resolution 3.40Å" /> '''RNA polymerase II el...)
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Revision as of 17:26, 20 November 2007
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RNA polymerase II elongation complex in 150 mm MG+2 with UTP
Overview
New structures of RNA polymerase II (pol II) transcribing complexes reveal, a likely key to transcription. The trigger loop swings beneath a correct, nucleoside triphosphate (NTP) in the nucleotide addition site, closing off, the active center and forming an extensive network of interactions with, the NTP base, sugar, phosphates, and additional pol II residues. A, histidine side chain in the trigger loop, precisely positioned by these, interactions, may literally "trigger" phosphodiester bond formation., Recognition and catalysis are thus coupled, ensuring the fidelity of, transcription.
About this Structure
2YU9 is a Protein complex structure of sequences from Saccharomyces cerevisiae with ZN, MG and UTP as ligands. This structure superseeds the now removed PDB entry 2NVS. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis., Wang D, Bushnell DA, Westover KD, Kaplan CD, Kornberg RD, Cell. 2006 Dec 1;127(5):941-54. PMID:17129781
Page seeded by OCA on Tue Nov 20 19:33:28 2007
