1p4k

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[[Image:1p4k.gif|left|200px]]
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{{STRUCTURE_1p4k| PDB=1p4k | SCENE= }}
{{STRUCTURE_1p4k| PDB=1p4k | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE GLYCOSYLASPARAGINASE PRECURSOR D151N MUTANT'''
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===CRYSTAL STRUCTURE OF THE GLYCOSYLASPARAGINASE PRECURSOR D151N MUTANT===
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==Overview==
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Glycosylasparaginase uses an autoproteolytic processing mechanism, through an N-O acyl shift, to generate a mature/active enzyme from a single-chain precursor. Structures of glycosylasparaginase precursors in complex with a glycine inhibitor have revealed the backbone in the immediate vicinity of the scissile peptide bond to be in a distorted trans conformation, which is believed to be the driving force for the N-O acyl shift to break the peptide bond. Here we report the effects of point mutation D151N. In addition to the loss of the base essential in autoproteolysis, this mutation also eradicates the backbone distortion near the scissile peptide bond. Binding of the glycine inhibitor to the autoproteolytic site of the D151N mutant does not restore the backbone distortion. Therefore, Asp151 plays a dual role, acting as the general base to activate the nucleophile and holding the distorted trans conformation that is critical for initiating an N-O acyl shift.
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(as it appears on PubMed at http://www.pubmed.gov), where 12906830 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12906830}}
==About this Structure==
==About this Structure==
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[[Category: Beta alpha]]
[[Category: Beta alpha]]
[[Category: Sandwich]]
[[Category: Sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:40:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:58:51 2008''

Revision as of 16:58, 27 July 2008

Image:1p4k.png

Template:STRUCTURE 1p4k

CRYSTAL STRUCTURE OF THE GLYCOSYLASPARAGINASE PRECURSOR D151N MUTANT

Template:ABSTRACT PUBMED 12906830

About this Structure

1P4K is a Single protein structure of sequence from Elizabethkingia meningoseptica. Full crystallographic information is available from OCA.

Reference

A dual role for an aspartic acid in glycosylasparaginase autoproteolysis., Qian X, Guan C, Guo HC, Structure. 2003 Aug;11(8):997-1003. PMID:12906830

Page seeded by OCA on Sun Jul 27 19:58:51 2008

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