2r0c

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[[Image:2r0c.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2r0c| PDB=2r0c | SCENE= }}
{{STRUCTURE_2r0c| PDB=2r0c | SCENE= }}
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'''Structure of the substrate-free form of the rebeccamycin biosynthetic enzyme REBC'''
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===Structure of the substrate-free form of the rebeccamycin biosynthetic enzyme REBC===
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==Overview==
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The biosynthesis of rebeccamycin, an antitumor compound, involves the remarkable eight-electron oxidation of chlorinated chromopyrrolic acid. Although one rebeccamycin biosynthetic enzyme is capable of generating low levels of the eight-electron oxidation product on its own, a second protein, RebC, is required to accelerate product formation and eliminate side reactions. However, the mode of action of RebC was largely unknown. Using crystallography, we have determined a likely function for RebC as a flavin hydroxylase, captured two snapshots of its dynamic catalytic cycle, and trapped a reactive molecule, a putative substrate, in its binding pocket. These studies strongly suggest that the role of RebC is to sequester a reactive intermediate produced by its partner protein and to react with it enzymatically, preventing its conversion to a suite of degradation products that includes, at low levels, the desired product.
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(as it appears on PubMed at http://www.pubmed.gov), where 17873060 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17873060}}
==About this Structure==
==About this Structure==
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[[Category: Monooxygenase]]
[[Category: Monooxygenase]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:01:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:02:54 2008''

Revision as of 17:02, 27 July 2008

Image:2r0c.png

Template:STRUCTURE 2r0c

Structure of the substrate-free form of the rebeccamycin biosynthetic enzyme REBC

Template:ABSTRACT PUBMED 17873060

About this Structure

2R0C is a Single protein structure of sequence from Lechevalieria aerocolonigenes. Full crystallographic information is available from OCA.

Reference

Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC., Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan CL, Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15311-6. Epub 2007 Sep 14. PMID:17873060

Page seeded by OCA on Sun Jul 27 20:02:54 2008

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