1hw6

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[[Image:1hw6.jpg|left|200px]]
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{{STRUCTURE_1hw6| PDB=1hw6 | SCENE= }}
{{STRUCTURE_1hw6| PDB=1hw6 | SCENE= }}
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'''CRYSTAL STRUCTURE OF APO-2,5-DIKETO-D-GLUCONATE REDUCTASE'''
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===CRYSTAL STRUCTURE OF APO-2,5-DIKETO-D-GLUCONATE REDUCTASE===
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==Overview==
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A 1.9 A resolution X-ray structure of the apo-form of Corynebacterium 2,5-diketo-d-gluconic acid reductase A (2,5-DKGR A), a member of the aldo-keto reductase superfamily, has been determined by molecular replacement using the NADPH-bound form of the same enzyme as the search model. 2,5-DKGR A catalyzes the NADPH-dependent stereo-specific reduction of 2,5-diketo-d-gluconate (2,5-DKG) to 2-keto-l-gulonate, a precursor in the industrial production of vitamin C. An atomic-resolution structure for the apo-form of the enzyme, in conjunction with our previously reported high-resolution X-ray structure for the holo-enzyme and holo/substrate model, allows a comparative analysis of structural changes that accompany cofactor binding. The results show that regions of the active site undergo coordinated conformational changes of up to 8 A. These conformational changes result in the organization and structural rearrangement of residues associated with substrate binding and catalysis. Thus, NADPH functions not only to provide a hydride ion for catalytic reduction, but is also a critical structural component for formation of a catalytically competent form of DKGR A.
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(as it appears on PubMed at http://www.pubmed.gov), where 11399090 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11399090}}
==About this Structure==
==About this Structure==
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[[Category: Aldo-keto reductase]]
[[Category: Aldo-keto reductase]]
[[Category: Tim barrel]]
[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:17:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:06:53 2008''

Revision as of 17:06, 27 July 2008

Image:1hw6.png

Template:STRUCTURE 1hw6

CRYSTAL STRUCTURE OF APO-2,5-DIKETO-D-GLUCONATE REDUCTASE

Template:ABSTRACT PUBMED 11399090

About this Structure

1HW6 is a Single protein structure of sequence from Corynebacterium sp.. Full crystallographic information is available from OCA.

Reference

Structural assembly of the active site in an aldo-keto reductase by NADPH cofactor., Sanli G, Blaber M, J Mol Biol. 2001 Jun 22;309(5):1209-18. PMID:11399090

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