1neq

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{{STRUCTURE_1neq| PDB=1neq | SCENE= }}
{{STRUCTURE_1neq| PDB=1neq | SCENE= }}
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'''SOLUTION STRUCTURE OF THE MU NER PROTEIN BY MULTIDIMENSIONAL NMR'''
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===SOLUTION STRUCTURE OF THE MU NER PROTEIN BY MULTIDIMENSIONAL NMR===
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==Overview==
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BACKGROUND: The Mu Ner protein is a small (74 amino acids), basic, DNA-binding protein found in phage Mu. It belongs to a class of proteins, the cro and repressor proteins, that regulate the switch from the lysogenic to the lytic state of the phage life cycle. There is no significant sequence identity between Mu Ner and the cro proteins of other phages, despite their functional similarity. In addition, there is no significant sequence identity with any other DNA-binding proteins, with the exception of Ner from the related phage D108 and the Nlp protein of Escherichia coli. As the tertiary structures of Mu Ner and these two related proteins are unknown, it is clear that a three-dimensional (3D) structure of Mu Ner is essential in order to gain insight into its mode of DNA binding. RESULTS: The 3D solution structure of Mu Ner has been solved by 3D and 4D heteronuclear magnetic resonance spectroscopy. The structure consists of five alpha helices, two of which comprise a helix-turn-helix (HTH) motif. Analysis of line broadening and disappearance of crosspeaks in a 1H-15N correlation spectrum of the Mu Ner-DNA complex suggests that residues in these two helices are most likely to be in contact with the DNA. CONCLUSIONS: Like the functionally analogous cro proteins from phages lambda and 434, the Mu Ner protein possesses a HTH DNA recognition motif. The Ner protein from phage D108 and the Nlp protein from E. coli are likely to have very similar tertiary structures due to high amino-acid-sequence identity with Mu Ner.
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(as it appears on PubMed at http://www.pubmed.gov), where 8590003 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8590003}}
==About this Structure==
==About this Structure==
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1NEQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_mu Enterobacteria phage mu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEQ OCA].
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1NEQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_mu Enterobacteria phage mu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEQ OCA].
==Reference==
==Reference==
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[[Category: Strzelecka, T E.]]
[[Category: Strzelecka, T E.]]
[[Category: Dna-binding protein]]
[[Category: Dna-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:26:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:12:04 2008''

Revision as of 17:12, 27 July 2008

Image:1neq.png

Template:STRUCTURE 1neq

SOLUTION STRUCTURE OF THE MU NER PROTEIN BY MULTIDIMENSIONAL NMR

Template:ABSTRACT PUBMED 8590003

About this Structure

1NEQ is a Single protein structure of sequence from Enterobacteria phage mu. Full experimental information is available from OCA.

Reference

The solution structure of the Mu Ner protein reveals a helix-turn-helix DNA recognition motif., Strzelecka TE, Clore GM, Gronenborn AM, Structure. 1995 Oct 15;3(10):1087-95. PMID:8590003

Page seeded by OCA on Sun Jul 27 20:12:04 2008

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