We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1rw4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1rw4.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1rw4.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1rw4| PDB=1rw4 | SCENE= }}
{{STRUCTURE_1rw4| PDB=1rw4 | SCENE= }}
-
'''Nitrogenase Fe protein l127 deletion variant'''
+
===Nitrogenase Fe protein l127 deletion variant===
-
==Overview==
+
<!--
-
The crystal structure of a nitrogenase Fe protein single site deletion variant reveals a distinctly new conformation of the Fe protein and indicates that, upon binding of MgATP, the Fe protein undergoes a dramatic conformational change that is largely manifested in the rigid-body reorientation of the homodimeric Fe protein subunits with respect to one another. The observed conformational state allows the rationalization of a model of structurally and chemically complementary interactions that occur upon initial complex formation with the MoFe protein component that are distinct from the protein-protein interactions that have been characterized previously for stabilized nitrogenase complexes. The crystallographic results, in combination with complementary UV-visible absorption, EPR, and resonance Raman spectroscopic data, indicate that the [4Fe-4S] cluster of both the Fe protein deletion variant and the native Fe protein in the presence of MgATP can reversibly cycle between a regular cubane-type [4Fe-4S] cluster in the reduced state and a cleaved form involving two [2Fe-2S] fragments in the oxidized state. Resonance Raman studies indicate that this novel cluster conversion is induced by glycerol, and the crystallographic data suggest that glycerol is bound as a bridging bidentate ligand to both [2Fe-2S] cluster fragments in the oxidized state.
+
The line below this paragraph, {{ABSTRACT_PUBMED_14967020}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 14967020 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_14967020}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Smith, A.]]
[[Category: Smith, A.]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:58:31 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:16:09 2008''

Revision as of 17:16, 27 July 2008

Image:1rw4.png

Template:STRUCTURE 1rw4

Nitrogenase Fe protein l127 deletion variant

Template:ABSTRACT PUBMED 14967020

About this Structure

1RW4 is a Single protein structure of sequence from Azotobacter vinelandii. Full crystallographic information is available from OCA.

Reference

A conformational mimic of the MgATP-bound "on state" of the nitrogenase iron protein., Sen S, Igarashi R, Smith A, Johnson MK, Seefeldt LC, Peters JW, Biochemistry. 2004 Feb 24;43(7):1787-97. PMID:14967020

Page seeded by OCA on Sun Jul 27 20:16:09 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rw4"
Personal tools