2fz5

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{{STRUCTURE_2fz5| PDB=2fz5 | SCENE= }}
{{STRUCTURE_2fz5| PDB=2fz5 | SCENE= }}
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'''Solution structure of two-electron reduced Megasphaera elsdenii flavodoxin'''
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===Solution structure of two-electron reduced Megasphaera elsdenii flavodoxin===
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==Overview==
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The tertiary structure of the non-crystallizable two-electron-reduced Megasphaera elsdenii flavodoxin (15 kDa, 137 amino acid residues) has been determined using nuclear Overhauser enhancement restraints extracted from two-dimensional 1H-NMR spectra. A tertiary structure satisfying the experimental restraints very well (maximum NOE violation of 66 pm) was obtained with use of restrained molecular dynamics, using 509 distance restraints (including one non-NOE) on a starting structure modeled from the crystal structure of one-electron-reduced Clostridium MP flavodoxin. The protein consists of a central parallel beta-sheet surrounded on both sides by two alpha-helices. The flavin is positioned at the periphery of the molecule. The tertiary structure of the protein is highly defined with the exception of the flavin. The latter is expected to result from performing the restrained molecular dynamics simulation without water molecules and without proper charges on the flavin. The flavin, including the phosphate, the ribityl side chain and the isoalloxazine ring, is solvent accessible under the experimental conditions used and evidenced by a two-dimensional amide exchange experiment. This accessibility is expected to be important in the redox potential regulation of the semiquinone/hydroquinone couple of the protein. The amide exchange against deuterons and several typical line shapes in the two-dimensional NMR spectra are consistent with the structure generated. The structure is discussed in detail.
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(as it appears on PubMed at http://www.pubmed.gov), where 2253614 is the PubMed ID number.
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{{ABSTRACT_PUBMED_2253614}}
==About this Structure==
==About this Structure==
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2FZ5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Megasphaera_elsdenii Megasphaera elsdenii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZ5 OCA].
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2FZ5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Megasphaera_elsdenii Megasphaera elsdenii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZ5 OCA].
==Reference==
==Reference==
Tertiary structure of two-electron reduced Megasphaera elsdenii flavodoxin and some implications, as determined by two-dimensional 1H-NMR and restrained molecular dynamics., van Mierlo CP, Lijnzaad P, Vervoort J, Muller F, Berendsen HJ, de Vlieg J, Eur J Biochem. 1990 Nov 26;194(1):185-98. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2253614 2253614]
Tertiary structure of two-electron reduced Megasphaera elsdenii flavodoxin and some implications, as determined by two-dimensional 1H-NMR and restrained molecular dynamics., van Mierlo CP, Lijnzaad P, Vervoort J, Muller F, Berendsen HJ, de Vlieg J, Eur J Biochem. 1990 Nov 26;194(1):185-98. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2253614 2253614]
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Secondary and tertiary structure characteristics of Megasphaera elsdenii flavodoxin in the reduced state as determined by two-dimensional 1H NMR., van Mierlo CP, Muller F, Vervoort J, Eur J Biochem. 1990 May 20;189(3):589-600. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2161759 2161759]
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A two-dimensional 1H NMR study on Megasphaera elsdenii flavodoxin in the reduced state. Sequential assignments., van Mierlo CP, Vervoort J, Muller F, Bacher A, Eur J Biochem. 1990 Feb 14;187(3):521-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2303055 2303055]
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A two-dimensional 1H-NMR study on Megasphaera elsdenii flavodoxin in the oxidized state and some comparisons with the two-electron-reduced state., van Mierlo CP, van der Sanden BP, van Woensel P, Muller F, Vervoort J, Eur J Biochem. 1990 Nov 26;194(1):199-216. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2253616 2253616]
[[Category: Megasphaera elsdenii]]
[[Category: Megasphaera elsdenii]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Alpha/beta doubly-wound topology]]
[[Category: Alpha/beta doubly-wound topology]]
[[Category: Non-covalently bound fmn]]
[[Category: Non-covalently bound fmn]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:17:03 2008''

Revision as of 17:17, 27 July 2008

Image:2fz5.png

Template:STRUCTURE 2fz5

Solution structure of two-electron reduced Megasphaera elsdenii flavodoxin

Template:ABSTRACT PUBMED 2253614

About this Structure

2FZ5 is a Single protein structure of sequence from Megasphaera elsdenii. Full experimental information is available from OCA.

Reference

Tertiary structure of two-electron reduced Megasphaera elsdenii flavodoxin and some implications, as determined by two-dimensional 1H-NMR and restrained molecular dynamics., van Mierlo CP, Lijnzaad P, Vervoort J, Muller F, Berendsen HJ, de Vlieg J, Eur J Biochem. 1990 Nov 26;194(1):185-98. PMID:2253614

Secondary and tertiary structure characteristics of Megasphaera elsdenii flavodoxin in the reduced state as determined by two-dimensional 1H NMR., van Mierlo CP, Muller F, Vervoort J, Eur J Biochem. 1990 May 20;189(3):589-600. PMID:2161759

A two-dimensional 1H NMR study on Megasphaera elsdenii flavodoxin in the reduced state. Sequential assignments., van Mierlo CP, Vervoort J, Muller F, Bacher A, Eur J Biochem. 1990 Feb 14;187(3):521-41. PMID:2303055

A two-dimensional 1H-NMR study on Megasphaera elsdenii flavodoxin in the oxidized state and some comparisons with the two-electron-reduced state., van Mierlo CP, van der Sanden BP, van Woensel P, Muller F, Vervoort J, Eur J Biochem. 1990 Nov 26;194(1):199-216. PMID:2253616

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