1nar

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[[Image:1nar.gif|left|200px]]
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{{Seed}}
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[[Image:1nar.png|left|200px]]
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{{STRUCTURE_1nar| PDB=1nar | SCENE= }}
{{STRUCTURE_1nar| PDB=1nar | SCENE= }}
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'''CRYSTAL STRUCTURE OF NARBONIN REFINED AT 1.8 ANGSTROMS RESOLUTION'''
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===CRYSTAL STRUCTURE OF NARBONIN REFINED AT 1.8 ANGSTROMS RESOLUTION===
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==Overview==
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The three-dimensional structure of narbonin, a seed protein from Vicia narbonensis L, has been determined at 1.8 A resolution. Phase information was obtained by multiple isomorphous replacement and optimized anomalous dispersion. The narbonin structure was initially traced with only 17% amino-acid sequence information and preliminarily refined to a crystallographic R-factor of 16.5%. It is now refined to 15.9% using full sequence information derived from cDNA and after the addition of more solvent molecules. The monomeric molecule of narbonin is an eight-stranded parallel beta-barrel surrounded by alpha-helices in a beta/alpha-topology similar to that first observed in triose phosphate isomerase. Differences exist in the N-terminal part of the polypeptide chain, where the first helix is replaced by a loop and the second beta-strand is followed by an additional antiparallel alpha-sheet placed parallel on top of alpha-helices alpha3 and alpha4. Two short additional secondary structures are present. The first, an alpha-helix, is situated between the seventh beta-strand and the following helix, and the second, which is a 3(10) helix, between the eighth strand and the C-terminal helix. The most striking observation is the lack of a known enzymatic function for narbonin, because all TIM-like structures known so far are enzymes.
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The line below this paragraph, {{ABSTRACT_PUBMED_15299319}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15299319 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15299319}}
==About this Structure==
==About this Structure==
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[[Category: Wilson, K S.]]
[[Category: Wilson, K S.]]
[[Category: Plant seed protein]]
[[Category: Plant seed protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:18:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:18:53 2008''

Revision as of 17:18, 27 July 2008

Image:1nar.png

Template:STRUCTURE 1nar

CRYSTAL STRUCTURE OF NARBONIN REFINED AT 1.8 ANGSTROMS RESOLUTION

Template:ABSTRACT PUBMED 15299319

About this Structure

1NAR is a Single protein structure of sequence from Vicia narbonensis. Full crystallographic information is available from OCA.

Reference

Crystal structure of narbonin at 1.8 A resolution., Hennig M, Pfeffer-Hennig S, Dauter Z, Wilson KS, Schlesier B, Nong VH, Acta Crystallogr D Biol Crystallogr. 1995 Mar 1;51(Pt 2):177-89. PMID:15299319

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