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1qj4

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[[Image:1qj4.jpg|left|200px]]
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{{STRUCTURE_1qj4| PDB=1qj4 | SCENE= }}
{{STRUCTURE_1qj4| PDB=1qj4 | SCENE= }}
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'''HYDROXYNITRILE-LYASE FROM HEVEA BRASILIENSIS AT ATOMIC RESOLUTION'''
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===HYDROXYNITRILE-LYASE FROM HEVEA BRASILIENSIS AT ATOMIC RESOLUTION===
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==Overview==
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The X-ray crystal structure of native hydroxynitrile lyase from Hevea brasiliensis (Hb-HNL) has been determined at 1.1 A resolution. It refined to a final R of 11.5% for all data and an Rfree of 14.4%. The favorable data-to-parameter ratio at atomic resolution made the refinement of individual anisotropic displacement parameters possible. The data also allowed a clear distinction of the alternate orientations of all histidine and the majority of asparagine and glutamine side chains. A number of hydrogen atoms, including one on the imidazole of the mechanistically important His-235, became visible as peaks in a difference electron density map. The structure revealed a discretely disordered sidechain of Ser-80, which is part of the putative catalytic triad. Analysis of the anisotropy indicated an increased mobility of residues near the entrance to the active site and within the active site.
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The line below this paragraph, {{ABSTRACT_PUBMED_10494852}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10494852 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10494852}}
==About this Structure==
==About this Structure==
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[[Category: Lyase]]
[[Category: Lyase]]
[[Category: Oxynitrilase]]
[[Category: Oxynitrilase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:20:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:19:04 2008''

Revision as of 17:19, 27 July 2008

Image:1qj4.png

Template:STRUCTURE 1qj4

HYDROXYNITRILE-LYASE FROM HEVEA BRASILIENSIS AT ATOMIC RESOLUTION

Template:ABSTRACT PUBMED 10494852

About this Structure

1QJ4 is a Single protein structure of sequence from Hevea brasiliensis. Full crystallographic information is available from OCA.

Reference

Atomic resolution crystal structure of hydroxynitrile lyase from Hevea brasiliensis., Gruber K, Gugganig M, Wagner UG, Kratky C, Biol Chem. 1999 Jul-Aug;380(7-8):993-1000. PMID:10494852

Page seeded by OCA on Sun Jul 27 20:19:04 2008

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