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| | {{STRUCTURE_3bn0| PDB=3bn0 | SCENE= }} | | {{STRUCTURE_3bn0| PDB=3bn0 | SCENE= }} |
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| - | '''The ribosomal protein S16 from Aquifex aeolicus'''
| + | ===The ribosomal protein S16 from Aquifex aeolicus=== |
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| - | ==Overview==
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| - | Understanding the mechanisms that dictate protein stability is of large relevance, for instance, to enable design of temperature-tolerant enzymes with high enzymatic activity over a broad temperature interval. In an effort to identify such mechanisms, we have performed a detailed comparative study of the folding thermodynamics and kinetics of the ribosomal protein S16 isolated from a mesophilic (S16(meso)) and hyperthermophilic (S16(thermo)) bacterium by using a variety of biophysical methods. As basis for the study, the 2.0 A X-ray structure of S16(thermo) was solved using single wavelength anomalous dispersion phasing. Thermal unfolding experiments yielded midpoints of 59 and 111 degrees C with associated changes in heat capacity upon unfolding (DeltaC(p)(0)) of 6.4 and 3.3 kJ mol(-1) K(-1), respectively. A strong linear correlation between DeltaC(p)(0) and melting temperature (T(m)) was observed for the wild-type proteins and mutated variants, suggesting that these variables are intimately connected. Stopped-flow fluorescence spectroscopy shows that S16(meso) folds through an apparent two-state model, whereas S16(thermo) folds through a more complex mechanism with a marked curvature in the refolding limb indicating the presence of a folding intermediate. Time-resolved energy transfer between Trp and N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-yl)methyl iodoacetamide of proteins mutated at selected positions shows that the denatured state ensemble of S16(thermo) is more compact relative to S16(meso). Taken together, our results suggest the presence of residual structure in the denatured state ensemble of S16(thermo) that appears to account for the large difference in quantified DeltaC(p)(0) values and, in turn, parts of the observed extreme thermal stability of S16(thermo). These observations may be of general importance in the design of robust enzymes that are highly active over a wide temperature span.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_18471828}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 18471828 is the PubMed ID number. |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Ribosomal protein]] | | [[Category: Ribosomal protein]] |
| | [[Category: Ribosome]] | | [[Category: Ribosome]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 28 09:19:50 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:28:37 2008'' |
Revision as of 17:28, 27 July 2008
Template:STRUCTURE 3bn0
The ribosomal protein S16 from Aquifex aeolicus
Template:ABSTRACT PUBMED 18471828
About this Structure
3BN0 is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Extreme Temperature Tolerance of a Hyperthermophilic Protein Coupled to Residual Structure in the Unfolded State., Wallgren M, Aden J, Pylypenko O, Mikaelsson T, Johansson LB, Rak A, Wolf-Watz M, J Mol Biol. 2008 Apr 8;. PMID:18471828
Page seeded by OCA on Sun Jul 27 20:28:37 2008
