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377d

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{{STRUCTURE_377d| PDB=377d | SCENE= }}
{{STRUCTURE_377d| PDB=377d | SCENE= }}
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'''5'-R(*CP*GP*UP*AP*CP*G)-3''''
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===5'-R(*CP*GP*UP*AP*CP*G)-3'===
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==Overview==
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The crystal structure of the alternating RNA r(CGUAC)dG with a 3'-terminal deoxy G residue has been determined at 1.76 A resolution. The crystal belongs to the orthorhombic space group C2221, unit-cell dimensions a = 29.53, b = 44.61 and c = 94.18 A, with two independent duplexes (I and II) per asymmetric unit. The structure was solved by the molecular-replacement method. The final R factor was 18.8% using 4757 reflections in the resolution range 8.0-1.76 A. The model contains a total of 496 atoms and 85 solvent molecules. The two duplexes form the repeating unit and stack in the usual head-to-tail (5',3'/5',3') fashion into a pseudocontinuous helical column. Almost all of the 2'-hydroxyl groups are engaged in the three modes of water-mediated interactions to the base N3/O2 atoms, the sugar O4' atoms and the backbone phosphates. Thus, the 2'-hydroxyl group of RNA is probably contributing to the stability of the duplexes.
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The line below this paragraph, {{ABSTRACT_PUBMED_9761851}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9761851 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9761851}}
==About this Structure==
==About this Structure==
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[[Category: A-rna]]
[[Category: A-rna]]
[[Category: Double helix]]
[[Category: Double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:17:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:31:55 2008''

Revision as of 17:32, 27 July 2008

Image:377d.png

Template:STRUCTURE 377d

5'-R(*CP*GP*UP*AP*CP*G)-3'

Template:ABSTRACT PUBMED 9761851

About this Structure

Full crystallographic information is available from OCA.

Reference

1.76 A structure of a pyrimidine start alternating A-RNA hexamer r(CGUAC)dG., Biswas R, Mitra SN, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):570-6. PMID:9761851

Page seeded by OCA on Sun Jul 27 20:31:55 2008

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