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2gcg

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{{STRUCTURE_2gcg| PDB=2gcg | SCENE= }}
{{STRUCTURE_2gcg| PDB=2gcg | SCENE= }}
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'''Ternary Crystal Structure of Human Glyoxylate Reductase/Hydroxypyruvate Reductase'''
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===Ternary Crystal Structure of Human Glyoxylate Reductase/Hydroxypyruvate Reductase===
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==Overview==
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Human glyoxylate reductase/hydroxypyruvate reductase (GRHPR) is a D-2-hydroxy-acid dehydrogenase that plays a critical role in the removal of the metabolic by-product glyoxylate from within the liver. Deficiency of this enzyme is the underlying cause of primary hyperoxaluria type 2 (PH2) and leads to increased urinary oxalate levels, formation of kidney stones and renal failure. Here we describe the crystal structure of human GRHPR at 2.2 A resolution. There are four copies of GRHPR in the crystallographic asymmetric unit: in each homodimer, one subunit forms a ternary (enzyme+NADPH+reduced substrate) complex, and the other a binary (enzyme+NADPH) form. The spatial arrangement of the two enzyme domains is the same in binary and ternary forms. This first crystal structure of a true ternary complex of an enzyme from this family demonstrates the relationship of substrate and catalytic residues within the active site, confirming earlier proposals of the mode of substrate binding, stereospecificity and likely catalytic mechanism for these enzymes. GRHPR has an unusual substrate specificity, preferring glyoxylate and hydroxypyruvate, but not pyruvate. A tryptophan residue (Trp141) from the neighbouring subunit of the dimer is projected into the active site region and appears to contribute to the selectivity for hydroxypyruvate. This first crystal structure of a human GRHPR enzyme also explains the deleterious effects of naturally occurring missense mutations of this enzyme that lead to PH2.
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==About this Structure==
==About this Structure==
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[[Category: Rumsby, G.]]
[[Category: Rumsby, G.]]
[[Category: Formate/glycerate dehydrogenase substrate-binding domain]]
[[Category: Formate/glycerate dehydrogenase substrate-binding domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:34:13 2008''

Revision as of 17:34, 27 July 2008

Image:2gcg.png

Template:STRUCTURE 2gcg

Ternary Crystal Structure of Human Glyoxylate Reductase/Hydroxypyruvate Reductase

Template:ABSTRACT PUBMED 16756993

About this Structure

2GCG is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis of substrate specificity in human glyoxylate reductase/hydroxypyruvate reductase., Booth MP, Conners R, Rumsby G, Brady RL, J Mol Biol. 2006 Jun 30;360(1):178-89. Epub 2006 May 22. PMID:16756993

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