2jni

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{{STRUCTURE_2jni| PDB=2jni | SCENE= }}
{{STRUCTURE_2jni| PDB=2jni | SCENE= }}
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'''Spatial structure of antimicrobial peptide arenicin-2 in aqueous solution'''
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===Spatial structure of antimicrobial peptide arenicin-2 in aqueous solution===
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==Overview==
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Arenicins are 21-residue cationic antimicrobial peptides, isolated from marine polychaeta Arenicola marina. In order to determine a high-resolution three-dimensional structure of arenicin-2, the recombinant peptide was overexpressed as a fused form in Escherichia coli. Both arenicin isoforms were synthesized using the Fmoc-based solid-phase strategy. Recombinant and synthetic arenicins were purified, and their antimicrobial and spectroscopic properties were analyzed. NMR investigation shows that in water solution arenicin-2 displays a prolonged beta-hairpin, formed by two antiparallel beta-strands and stabilized by one disulfide and nine hydrogen bonds. A significant right-handed twist in the beta-sheet is deprived the peptide surface of amphipathicity. CD spectroscopic analysis indicates that arenicin-2 binds to the SDS and DPC micelles, and conformation of the peptide is significantly changed upon binding. Arenicin strongly binds to anionic lipid (POPE/POPG) vesicles in contrast with zwitterionic (POPC) ones. These results suggest that arenicins are membrane active peptides and point to possible mechanism of their selectivity toward bacterial cells.
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{{ABSTRACT_PUBMED_17585874}}
==About this Structure==
==About this Structure==
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2JNI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arenicola_marina Arenicola marina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNI OCA].
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2JNI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arenicola_marina Arenicola marina]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNI OCA].
==Reference==
==Reference==
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[[Category: Beta-sheet]]
[[Category: Beta-sheet]]
[[Category: Peptide]]
[[Category: Peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:35:38 2008''

Revision as of 17:35, 27 July 2008

Image:2jni.png

Template:STRUCTURE 2jni

Spatial structure of antimicrobial peptide arenicin-2 in aqueous solution

Template:ABSTRACT PUBMED 17585874

About this Structure

2JNI is a Single protein structure of sequence from Arenicola marina. Full experimental information is available from OCA.

Reference

Recombinant expression, synthesis, purification, and solution structure of arenicin., Ovchinnikova TV, Shenkarev ZO, Nadezhdin KD, Balandin SV, Zhmak MN, Kudelina IA, Finkina EI, Kokryakov VN, Arseniev AS, Biochem Biophys Res Commun. 2007 Aug 17;360(1):156-62. Epub 2007 Jun 14. PMID:17585874

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