1n4l

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{{STRUCTURE_1n4l| PDB=1n4l | SCENE= }}
{{STRUCTURE_1n4l| PDB=1n4l | SCENE= }}
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'''A DNA analogue of the polypurine tract of HIV-1'''
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===A DNA analogue of the polypurine tract of HIV-1===
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==Overview==
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The polypurine tract (PPT) from the HIV-1 genome is resistant to digestion by reverse transcriptase following (-)-strand synthesis and is used to prime (+)-strand synthesis during retroviral replication. We have determined the crystal structure of the asymmetric DNA/DNA analog16-mer duplex (CTTTTTAAAAGAAAAG/CTTTTCTTTTAAAAAG) comprising most of the "visible" portion of the RNA:DNA hybrid from the polypurine tract of HIV-1, which was previously reported in a complex with HIV-1 reverse transcriptase. Our 16-mer completely encompasses a 10-mer DNA duplex analog of the HIV-1 PPT. We report here a detailed analysis of our B' form 16-mer DNA structure, including three full pure A-tracts, as well as a comparative structural analysis with polypurine tract and other A-tract-containing nucleic acid structures. Our analysis reveals that the polypurine tract structures share structural features despite being different nucleic acid forms (i.e. DNA:DNA versus RNA:DNA). In addition, the previously reported A-tract-containing DNA molecules bound to topoisomerase I are remarkably similar to our polypurine tract 16-mer structure. On the basis of our analysis, we suggest that the specific topology of long pure A-tracts is remarkably comparable across a wide array of biological environments.
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(as it appears on PubMed at http://www.pubmed.gov), where 12818202 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12818202}}
==About this Structure==
==About this Structure==
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[[Category: Polypurine tract]]
[[Category: Polypurine tract]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:05:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:36:52 2008''

Revision as of 17:36, 27 July 2008

Image:1n4l.png

Template:STRUCTURE 1n4l

A DNA analogue of the polypurine tract of HIV-1

Template:ABSTRACT PUBMED 12818202

About this Structure

1N4L is a Single protein structure of sequence from Moloney murine leukemia virus. Full crystallographic information is available from OCA.

Reference

Staying straight with A-tracts: a DNA analog of the HIV-1 polypurine tract., Cote ML, Pflomm M, Georgiadis MM, J Mol Biol. 2003 Jun 27;330(1):57-74. PMID:12818202

Page seeded by OCA on Sun Jul 27 20:36:52 2008

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