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1p6b

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[[Image:1p6b.jpg|left|200px]]
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{{STRUCTURE_1p6b| PDB=1p6b | SCENE= }}
{{STRUCTURE_1p6b| PDB=1p6b | SCENE= }}
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'''X-ray structure of phosphotriesterase, triple mutant H254G/H257W/L303T'''
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===X-ray structure of phosphotriesterase, triple mutant H254G/H257W/L303T===
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==Overview==
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The bacterial phosphotriesterase has been utilized as a template for the evolution of improved enzymes for the catalytic decomposition of organophosphate nerve agents. A combinatorial library of active site mutants was constructed by randomizing residues His-254, His-257, and Leu-303. The collection of mutant proteins was screened for the ability to hydrolyze a chromogenic analogue of the most toxic stereoisomer of the chemical warfare agent, soman. The mutant H254G/H257W/L303T catalyzed the hydrolysis of the target substrate nearly 3 orders of magnitude faster than the wild-type enzyme. The X-ray crystal structure was solved in the presence and absence of diisopropyl methyl phosphonate. The mutant enzyme was ligated to an additional divalent cation at the active site that was displaced upon the binding of the substrate analogue inhibitor. These studies demonstrate that substantial changes in substrate specificity can be achieved by relatively minor changes to the primary amino acid sequence.
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(as it appears on PubMed at http://www.pubmed.gov), where 15369336 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15369336}}
==About this Structure==
==About this Structure==
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[[Category: Nerve agent]]
[[Category: Nerve agent]]
[[Category: Tim barrel]]
[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:44:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:37:42 2008''

Revision as of 17:37, 27 July 2008

Image:1p6b.png

Template:STRUCTURE 1p6b

X-ray structure of phosphotriesterase, triple mutant H254G/H257W/L303T

Template:ABSTRACT PUBMED 15369336

About this Structure

1P6B is a Single protein structure of sequence from Flavobacterium sp.. Full crystallographic information is available from OCA.

Reference

Enhanced degradation of chemical warfare agents through molecular engineering of the phosphotriesterase active site., Hill CM, Li WS, Thoden JB, Holden HM, Raushel FM, J Am Chem Soc. 2003 Jul 30;125(30):8990-1. PMID:15369336

Page seeded by OCA on Sun Jul 27 20:37:42 2008

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