2c35

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{{STRUCTURE_2c35| PDB=2c35 | SCENE= }}
{{STRUCTURE_2c35| PDB=2c35 | SCENE= }}
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'''SUBUNITS RPB4 AND RPB7 OF HUMAN RNA POLYMERASE II'''
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===SUBUNITS RPB4 AND RPB7 OF HUMAN RNA POLYMERASE II===
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==Overview==
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The Rpb4 and Rpb7 subunits of eukaryotic RNA polymerase II (RNAP(II)) form a heterodimer that protrudes from the 10-subunit core of the enzyme. We have obtained crystals of the human Rpb4/Rpb7 heterodimer and determined the structure to 2.7 A resolution. The presence of putative RNA-binding domains on the Rpb7 subunit and the position of the heterodimer close to the RNA exit groove in the 12 subunit yeast polymerase complex strongly suggests a role for the heterodimer in binding and stabilizing the nascent RNA transcript. We have complemented the structural analysis with biochemical studies directed at dissecting the RNA-binding properties of the human Rpb4/Rpb7 complex and that of the homologous E/F complex from Methanocaldococcus jannaschii. A number of conserved, solvent-exposed residues in both the human Rpb7 subunit and the archaeal E subunit have been modified by site-directed mutagenesis and the mutants tested for RNA binding by performing electrophoretic mobility shift assays. These studies have identified an elongated surface region on the corresponding face of both subunit E and Rpb7 that is involved in RNA binding. The area spans the nucleic acid binding face of the OB fold, including the B4-B5 loop, but also extends towards the N-terminal domain.
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{{ABSTRACT_PUBMED_16282592}}
==About this Structure==
==About this Structure==
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[[Category: Rna polymerase ii]]
[[Category: Rna polymerase ii]]
[[Category: Transcription]]
[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:10:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:38:34 2008''

Revision as of 17:38, 27 July 2008

Image:2c35.png

Template:STRUCTURE 2c35

SUBUNITS RPB4 AND RPB7 OF HUMAN RNA POLYMERASE II

Template:ABSTRACT PUBMED 16282592

About this Structure

2C35 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II., Meka H, Werner F, Cordell SC, Onesti S, Brick P, Nucleic Acids Res. 2005 Nov 10;33(19):6435-44. Print 2005. PMID:16282592

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