1kqj

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spinqualitylabelsall models 1kqj, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of a Mutant of MutY Catalytic Domain

Overview

The Escherichia coli DNA repair enzyme MutY plays an important role in the, recognition and repair of, 7,8-dihydro-8-oxo-2'-deoxyguanosine-2'-deoxyadenosine (OG*A) mismatches in, DNA. MutY prevents DNA mutations caused by the misincorporation of A, opposite OG by catalyzing the deglycosylation of the aberrant adenine., MutY is representative of a unique subfamily of DNA repair enzymes that, also contain a [4Fe-4S]2+ cluster, which has been implicated in substrate, recognition. Previously, we have used site-directed mutagenesis to, individually replace the cysteine ligands to the [4Fe-4S]2+ cluster of E., coli MutY with serine, histidine, or alanine. These experiments suggested, that histidine coordination to the iron-sulfur cluster may be accommodated, in MutY at position 199. Purification and enzymatic analysis of C199H and, C199S forms indicated that these forms behave nearly identical to the WT, enzyme. Furthermore, introduction of the C199H mutation in a truncated, form of MutY (C199HT) allowed for crystallization and structural, characterization of the modified [4Fe-4S] cluster coordination. The C199HT, structure showed that histidine coordinated to the iron cluster although, comparison to the structure of the WT truncated enzyme indicated that the, occupancy of iron at the modified position had been reduced to 60%., Electron paramagnetic resonance (EPR) spectroscopy on samples of C199HT, indicates that a significant percentage (15-30%) of iron clusters were of, the [3Fe-4S]1+ form. Oxidation of the C199HT enzyme with ferricyanide, increases the amount of the 3Fe cluster by approximately 2-fold. Detailed, kinetic analysis on samples containing a mixture of [3Fe-4S]1+ and, [4Fe-4S]2+ forms indicated that the reactivity of the [3Fe-4S]1+ C199HT, enzyme does not differ significantly from that of the WT truncated enzyme., The relative resistance of the [4Fe-4S]2+ cluster toward oxidation, as, well as the retention of activity of the [3Fe-4S]1+ form, may be an, important aspect of the role of MutY in repair of DNA damage resulting, from oxidative stress.

About this Structure

1KQJ is a Single protein structure of sequence from Escherichia coli with SO4, SF4 and GOL as ligands. Full crystallographic information is available from OCA.

Reference

Noncysteinyl coordination to the [4Fe-4S]2+ cluster of the DNA repair adenine glycosylase MutY introduced via site-directed mutagenesis. Structural characterization of an unusual histidinyl-coordinated cluster., Messick TE, Chmiel NH, Golinelli MP, Langer MR, Joshua-Tor L, David SS, Biochemistry. 2002 Mar 26;41(12):3931-42. PMID:11900536

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