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| | {{STRUCTURE_2g0f| PDB=2g0f | SCENE= }} | | {{STRUCTURE_2g0f| PDB=2g0f | SCENE= }} |
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| - | '''Crystal Structure of P144A mutant of E.coli CcmG protein'''
| + | ===Crystal Structure of P144A mutant of E.coli CcmG protein=== |
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| - | ==Overview==
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| - | CcmG, also designated DsbE, functions as a periplasmic protein thiol:disulfide oxidoreductase and is required for cytochrome c maturation. Here we report the crystal structures of Escherichia coli CcmG and its two mutants, P144A and the N-terminal fifty seven-residue deletion mutant, and two additional deletion mutants were studied by circular dichroism. Structural comparison of E. coli CcmG with its deletion mutants reveals that the N-terminal beta-sheet is essential for maintaining the folding topology and consequently maintaining the active-site structure of CcmG. Pro144 and Glu145 are key residues of the fingerprint region of CcmG. Pro144 is in cis-configuration, and it makes van der Waals interactions with the active-site disulfide Cys80-Cys83 and forms a C--H...O hydrogen bond with Thr82, helping stabilize the active-site structure. Glu145 forms a salt-bridge and hydrogen-bond network with other residues of the fingerprint region and with Arg158, further stabilizing the active-site structure. The cis-configuration of Pro144 makes the backbone nitrogen and oxygen of Ala143 exposed to solvent, favorable for interacting with binding partners. The key role of cis-Pro144 is verified by the P144A mutant, which contains trans-Ala144 and displays redox property changes. Structural comparison of E. coli CcmG with the recently reported structure of CcmG in complex with the N-terminal domain of DsbD reveals that Tyr141 undergoes conformational changes upon binding DsbD. A cis-proline located at the N-terminus of the first beta-strand of the betabetaalpha motif of the thioredoxin-like domain is a conserved structural feature of the thioredoxin superfamily.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17019698}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17019698 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17019698}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: E coli ccmg]] | | [[Category: E coli ccmg]] |
| | [[Category: P144a mutant]] | | [[Category: P144a mutant]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:33:21 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:57:41 2008'' |
Revision as of 17:57, 27 July 2008
Template:STRUCTURE 2g0f
Crystal Structure of P144A mutant of E.coli CcmG protein
Template:ABSTRACT PUBMED 17019698
About this Structure
2G0F is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structures of E. coli CcmG and its mutants reveal key roles of the N-terminal beta-sheet and the fingerprint region., Ouyang N, Gao YG, Hu HY, Xia ZX, Proteins. 2006 Dec 1;65(4):1021-31. PMID:17019698
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