2a3g

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{{STRUCTURE_2a3g| PDB=2a3g | SCENE= }}
{{STRUCTURE_2a3g| PDB=2a3g | SCENE= }}
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'''The structure of T6 bovine insulin'''
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===The structure of T6 bovine insulin===
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==Overview==
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Porcine insulin differs in sequence from bovine insulin at residues A8 (Thr in porcine--&gt;Ala in bovine) and A10 (Ile in porcine--&gt;Val in bovine). The structure of T6 hexameric bovine insulin has been determined to 2.25 A resolution at room temperature and refined to a residual of 0.162. The structure of the independent dimer is nearly identical to the T6 porcine insulin dimer: the mean displacement of all backbone atoms is 0.16 A, with the largest displacements occurring at AlaB30. Each of two independent zinc ions is octahedrally coordinated by three HisB10 side chains and three water molecules. As has been observed in both human and porcine insulin, the GluB13 side chains are directed towards the center of the hexamer, where a short contact of 2.57 A occurs between two independent carboxyl O atoms, again suggesting the presence of a centered hydrogen bond. No significant displacements of backbone atoms or changes in conformation are observed at A8 or A10. Since there are no interhexamer hydrogen-bonded contacts involving A8 in either porcine or bovine insulin, the change in the identity of this residue appears to have little or no effect upon the packing of the hexamers in the unit cell. In contrast, the side chains of the three A10 residues in one trimer make van der Waals contacts with the A10 side chains in a translationally related hexamer. As a consequence of the loss of the C(delta1) atom from the isoleucine residue in porcine insulin to produce valine in bovine insulin, there is a 0.36 A decrease in the distance between independent pairs of C(beta) atoms and a 0.24 A decrease in the c dimension of the unit cell. Thus, the net effect of the change in sequence at A10 is to strengthen the stabilizing hydrophobic interactions between hexamers.
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(as it appears on PubMed at http://www.pubmed.gov), where 16239724 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16239724}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
The structure of T6 bovine insulin., Smith GD, Pangborn WA, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1476-82. Epub 2005, Oct 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16239724 16239724]
The structure of T6 bovine insulin., Smith GD, Pangborn WA, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1476-82. Epub 2005, Oct 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16239724 16239724]
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The structure of 2Zn pig insulin crystals at 1.5 A resolution., Baker EN, Blundell TL, Cutfield JF, Cutfield SM, Dodson EJ, Dodson GG, Hodgkin DM, Hubbard RE, Isaacs NW, Reynolds CD, et al., Philos Trans R Soc Lond B Biol Sci. 1988 Jul 6;319(1195):369-456. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2905485 2905485]
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The structure of T6 human insulin at 1.0 A resolution., Smith GD, Pangborn WA, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):474-82. Epub 2003, Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12595704 12595704]
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Lessons from an aged, dried crystal of T(6) human insulin., Smith GD, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1384-94. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876340 12876340]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Insulin family]]
[[Category: Insulin family]]
[[Category: Signal]]
[[Category: Signal]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:33:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:00:44 2008''

Revision as of 18:00, 27 July 2008

Image:2a3g.png

Template:STRUCTURE 2a3g

The structure of T6 bovine insulin

Template:ABSTRACT PUBMED 16239724

About this Structure

2A3G is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

The structure of T6 bovine insulin., Smith GD, Pangborn WA, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1476-82. Epub 2005, Oct 19. PMID:16239724

The structure of 2Zn pig insulin crystals at 1.5 A resolution., Baker EN, Blundell TL, Cutfield JF, Cutfield SM, Dodson EJ, Dodson GG, Hodgkin DM, Hubbard RE, Isaacs NW, Reynolds CD, et al., Philos Trans R Soc Lond B Biol Sci. 1988 Jul 6;319(1195):369-456. PMID:2905485

The structure of T6 human insulin at 1.0 A resolution., Smith GD, Pangborn WA, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):474-82. Epub 2003, Feb 21. PMID:12595704

Lessons from an aged, dried crystal of T(6) human insulin., Smith GD, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1384-94. Epub 2003, Jul 23. PMID:12876340

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