1pjf

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{{STRUCTURE_1pjf| PDB=1pjf | SCENE= }}
{{STRUCTURE_1pjf| PDB=1pjf | SCENE= }}
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'''Solid State NMR structure of the Pf1 Major Coat Protein in Magnetically Aligned Bacteriophage'''
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===Solid State NMR structure of the Pf1 Major Coat Protein in Magnetically Aligned Bacteriophage===
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==Overview==
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The atomic resolution structure of Pf1 coat protein determined by solid-state NMR spectroscopy of magnetically aligned filamentous bacteriophage particles in solution is compared to the structures previously determined by X-ray fiber and neutron diffraction, the structure of its membrane-bound form, and the structure of fd coat protein. These structural comparisons provide insights into several biological properties, differences between class I and class II filamentous bacteriophages, and the assembly process. The six N-terminal amino acid residues adopt an unusual "double hook" conformation on the outside of the bacteriophage particle. The solid-state NMR results indicate that at 30 degrees C, some of the coat protein subunits assume a single, fully structured conformation, and some have a few mobile residues that provide a break between two helical segments, in agreement with structural models from X-ray fiber and neutron diffraction, respectively. The atomic resolution structure determined by solid-state NMR for residues 7-14 and 18-46, which excludes the N-terminal double hook and the break between the helical segments, but encompasses more than 80% of the backbone including the distinct kink at residue 29, agrees with that determined by X-ray fiber diffraction with an RMSD value of 2.0 A. The symmetry and distance constraints determined by X-ray fiber and neutron diffraction enable the construction of an accurate model of the bacteriophage particle from the coordinates of the coat protein monomers.
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{{ABSTRACT_PUBMED_15288792}}
==About this Structure==
==About this Structure==
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1PJF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf1 Pseudomonas phage pf1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJF OCA].
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1PJF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf1 Pseudomonas phage pf1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJF OCA].
==Reference==
==Reference==
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[[Category: Helical virus]]
[[Category: Helical virus]]
[[Category: Virus/viral protein]]
[[Category: Virus/viral protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:09:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:06:21 2008''

Revision as of 18:06, 27 July 2008

Template:STRUCTURE 1pjf

Solid State NMR structure of the Pf1 Major Coat Protein in Magnetically Aligned Bacteriophage

Template:ABSTRACT PUBMED 15288792

About this Structure

1PJF is a Single protein structure of sequence from Pseudomonas phage pf1. Full experimental information is available from OCA.

Reference

Structure of the coat protein in Pf1 bacteriophage determined by solid-state NMR spectroscopy., Thiriot DS, Nevzorov AA, Zagyanskiy L, Wu CH, Opella SJ, J Mol Biol. 2004 Aug 13;341(3):869-79. PMID:15288792

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