1n9k

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[[Image:1n9k.png|left|200px]]
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{{STRUCTURE_1n9k| PDB=1n9k | SCENE= }}
{{STRUCTURE_1n9k| PDB=1n9k | SCENE= }}
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'''Crystal structure of the bromide adduct of AphA class B acid phosphatase/phosphotransferase from E. coli at 2.2 A resolution'''
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===Crystal structure of the bromide adduct of AphA class B acid phosphatase/phosphotransferase from E. coli at 2.2 A resolution===
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==Overview==
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AphA is a periplasmic acid phosphatase of Escherichia coli belonging to class B bacterial phosphatases, which is part of the DDDD superfamily of phosphohydrolases. The crystal structure of AphA has been determined at 2.2A and its resolution extended to 1.7A on an AuCl(3) derivative. This represents the first crystal structure of a class B bacterial phosphatase. Despite the lack of sequence homology, the AphA structure reveals a haloacid dehalogenase-like fold. This finding suggests that this fold could be conserved among members of the DDDD superfamily of phosphohydrolases. The active enzyme is a homotetramer built by using an extended N-terminal arm intertwining the four monomers. The active site of the native enzyme, as prepared, hosts a magnesium ion, which can be replaced by other metal ions. The structure explains the non-specific behaviour of AphA towards substrates, while a structure-based alignment with other phosphatases provides clues about the catalytic mechanism.
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The line below this paragraph, {{ABSTRACT_PUBMED_14687572}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 14687572 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14687572}}
==About this Structure==
==About this Structure==
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[[Category: Metallo-enzyme bromide mad]]
[[Category: Metallo-enzyme bromide mad]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 04:51:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:15:01 2008''

Revision as of 18:15, 27 July 2008

Image:1n9k.png

Template:STRUCTURE 1n9k

Crystal structure of the bromide adduct of AphA class B acid phosphatase/phosphotransferase from E. coli at 2.2 A resolution

Template:ABSTRACT PUBMED 14687572

About this Structure

1N9K is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The first structure of a bacterial class B Acid phosphatase reveals further structural heterogeneity among phosphatases of the haloacid dehalogenase fold., Calderone V, Forleo C, Benvenuti M, Cristina Thaller M, Maria Rossolini G, Mangani S, J Mol Biol. 2004 Jan 16;335(3):761-73. PMID:14687572

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