2hgn

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[[Image:2hgn.gif|left|200px]]
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{{STRUCTURE_2hgn| PDB=2hgn | SCENE= }}
{{STRUCTURE_2hgn| PDB=2hgn | SCENE= }}
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'''NMR structure of the third qRRM domain of human hnRNP F'''
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===NMR structure of the third qRRM domain of human hnRNP F===
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==Overview==
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The heterogeneous nuclear ribonucleoprotein (hnRNP) F belongs to the hnRNP H family involved in the regulation of alternative splicing and polyadenylation and specifically recognizes poly(G) sequences (G-tracts). In particular, hnRNP F binds a G-tract of the Bcl-x RNA and regulates its alternative splicing, leading to two isoforms, Bcl-x(S) and Bcl-x(L), with antagonist functions. In order to gain insight into G-tract recognition by hnRNP H members, we initiated an NMR study of human hnRNP F. We present the solution structure of the three quasi RNA recognition motifs (qRRMs) of hnRNP F and identify the residues that are important for the interaction with the Bcl-x RNA by NMR chemical shift perturbation and mutagenesis experiments. The three qRRMs exhibit the canonical betaalphabetabetaalphabeta RRM fold but additional secondary structure elements are present in the two N-terminal qRRMs of hnRNP F. We show that qRRM1 and qRRM2 but not qRRM3 are responsible for G-tract recognition and that the residues of qRRM1 and qRRM2 involved in G-tract interaction are not on the beta-sheet surface as observed for the classical RRM but are part of a short beta-hairpin and two adjacent loops. These regions define a novel interaction surface for RNA recognition by RRMs.
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{{ABSTRACT_PUBMED_16885237}}
==About this Structure==
==About this Structure==
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2HGN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HGN OCA].
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2HGN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HGN OCA].
==Reference==
==Reference==
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[[Category: G-tract]]
[[Category: G-tract]]
[[Category: Rna recognition motif]]
[[Category: Rna recognition motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:16:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:18:09 2008''

Revision as of 18:18, 27 July 2008

Image:2hgn.png

Template:STRUCTURE 2hgn

NMR structure of the third qRRM domain of human hnRNP F

Template:ABSTRACT PUBMED 16885237

About this Structure

2HGN is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition., Dominguez C, Allain FH, Nucleic Acids Res. 2006 Aug 2;34(13):3634-45. Print 2006. PMID:16885237

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