2o9l

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{{STRUCTURE_2o9l| PDB=2o9l | SCENE= }}
{{STRUCTURE_2o9l| PDB=2o9l | SCENE= }}
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'''AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element'''
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===AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element===
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==Overview==
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The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike sigma(70)-type proteins, the alternative sigma factor, sigma(54), requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of sigma(54) bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients sigma(54) on the promoter, and suggests how the C-terminal domain of sigma(54) interacts with RNAP.
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{{ABSTRACT_PUBMED_17481658}}
==About this Structure==
==About this Structure==
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2O9L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O9L OCA].
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2O9L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O9L OCA].
==Reference==
==Reference==
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[[Category: Sigma-54]]
[[Category: Sigma-54]]
[[Category: Transcription factor]]
[[Category: Transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:29:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:30:41 2008''

Revision as of 18:30, 27 July 2008

Image:2o9l.png

Template:STRUCTURE 2o9l

AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element

Template:ABSTRACT PUBMED 17481658

About this Structure

2O9L is a Single protein structure of sequence from Aquifex aeolicus. Full experimental information is available from OCA.

Reference

Structural basis of DNA recognition by the alternative sigma-factor, sigma54., Doucleff M, Pelton JG, Lee PS, Nixon BT, Wemmer DE, J Mol Biol. 2007 Jun 15;369(4):1070-8. Epub 2007 Apr 12. PMID:17481658

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