1mua

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{{STRUCTURE_1mua| PDB=1mua | SCENE= }}
{{STRUCTURE_1mua| PDB=1mua | SCENE= }}
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'''STRUCTURE AND ENERGETICS OF A NON-PROLINE CIS-PEPTIDYL LINKAGE IN AN ENGINEERED PROTEIN'''
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===STRUCTURE AND ENERGETICS OF A NON-PROLINE CIS-PEPTIDYL LINKAGE IN AN ENGINEERED PROTEIN===
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==Overview==
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The crystal structure of a human carbonic anhydrase II (CAII) variant, cis-proline-202--&gt;alanine (P202A), has been determined at 1.7-A resolution, indicating that the wild-type geometry, including the cis-peptidyl linkage, is retained upon substitution of proline by alanine. The CO2 hydrase activity and affinity for sulfonamide inhibitors of P202A CAII are virtually identical to those of wild type. However, the substitution of cis-alanine for cis-proline decreases the stability of the folded state by approximately 5 kcal mol-1 relative to both the unfolded state and an equilibrium intermediate in guanidine hydrochloride-induced denaturation. This destabilization can be attributed mainly to the less favorable cis/trans equilibrium of Xaa-alanine bonds compared to Xaa-proline bonds in the denatured state although other factors, including increased conformational entropy of the denatured state and decreased packing interactions in the native state, also contribute to the observed destabilization. The high catalytic activity of P202A CAII illustrates that unfavorable local conformations are nonetheless endured to satisfy the precise structural requirements of catalysis and ligand binding in the CAII active site.
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(as it appears on PubMed at http://www.pubmed.gov), where 8218160 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8218160}}
==About this Structure==
==About this Structure==
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[[Category: Christianson, D W.]]
[[Category: Christianson, D W.]]
[[Category: Nair, S K.]]
[[Category: Nair, S K.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:43:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:41:11 2008''

Revision as of 18:41, 27 July 2008

Image:1mua.png

Template:STRUCTURE 1mua

STRUCTURE AND ENERGETICS OF A NON-PROLINE CIS-PEPTIDYL LINKAGE IN AN ENGINEERED PROTEIN

Template:ABSTRACT PUBMED 8218160

About this Structure

1MUA is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure and energetics of a non-proline cis-peptidyl linkage in a proline-202-->alanine carbonic anhydrase II variant., Tweedy NB, Nair SK, Paterno SA, Fierke CA, Christianson DW, Biochemistry. 1993 Oct 19;32(41):10944-9. PMID:8218160

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