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2c7g

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[[Image:2c7g.gif|left|200px]]
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{{STRUCTURE_2c7g| PDB=2c7g | SCENE= }}
{{STRUCTURE_2c7g| PDB=2c7g | SCENE= }}
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'''FPRA FROM MYCOBACTERIUM TUBERCULOSIS: HIS57GLN MUTANT'''
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===FPRA FROM MYCOBACTERIUM TUBERCULOSIS: HIS57GLN MUTANT===
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==Overview==
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Mycobacterium tuberculosis FprA is a NADPH-ferredoxin reductase, functionally and structurally similar to the mammalian adrenodoxin reductase. It is presumably involved in supplying electrons to one or more of the pathogen's cytochrome P450s through reduced ferredoxins. It has been proposed on the basis of crystallographic data (Bossi, R. T., et al. (2002) Biochemistry 41, 8807-8818) that the highly conserved His57 and Glu214 whose side chains are H-bonded are involved in catalysis. Both residues were individually changed to nonionizable amino acyl residues through site-directed mutagenesis. Steady-state kinetics showed that the role of Glu214 in catalysis is negligible. On the contrary, the substitutions of His57 markedly impaired the catalytic efficiency of FprA for ferredoxin in the physiological reaction. Furthemore, they decreased the k(cat)/K(m) value for NADPH in the ferricyanide reduction. Rapid-reaction (stopped-flow) kinetic analysis of the isolated reductive half-reaction of wild-type and His57Gln forms of FprA with NADPH and NADH allowed a detailed description of the mechanism of enzyme-bound FAD reduction, with the identification of the intermediates involved. The His57Gln mutation caused a 6-fold decrease in the rate of hydride transfer from either NADPH or NADH to the enzyme-bound FAD cofactor. The 3D structure of FprA-H57Q, obtained at 1.8 A resolution, explains the inefficient hydride transfer of the mutant in terms of a suboptimal geometry of the nicotinamide-isoalloxazine interaction in the active site. These data demonstrate the role of His57 in the correct binding of NADPH to FprA for the subsequent steps of the catalytic cycle to proceed at a high rate.
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(as it appears on PubMed at http://www.pubmed.gov), where 16846214 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16846214}}
==About this Structure==
==About this Structure==
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Tuberculosis]]
[[Category: Tuberculosis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:43:41 2008''

Revision as of 18:43, 27 July 2008

Image:2c7g.png

Template:STRUCTURE 2c7g

FPRA FROM MYCOBACTERIUM TUBERCULOSIS: HIS57GLN MUTANT

Template:ABSTRACT PUBMED 16846214

About this Structure

2C7G is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Role of the His57-Glu214 ionic couple located in the active site of Mycobacterium tuberculosis FprA., Pennati A, Razeto A, de Rosa M, Pandini V, Vanoni MA, Mattevi A, Coda A, Aliverti A, Zanetti G, Biochemistry. 2006 Jul 25;45(29):8712-20. PMID:16846214

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