2tsb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2tsb.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2tsb.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2tsb| PDB=2tsb | SCENE= }}
{{STRUCTURE_2tsb| PDB=2tsb | SCENE= }}
-
'''AZURIN MUTANT M121A-AZIDE'''
+
===AZURIN MUTANT M121A-AZIDE===
-
==Overview==
+
<!--
-
The crystal structures of the azurin mutant Met121Ala and its azide derivative Met121Ala-azide from Pseudomonas aeruginosa have been determined. The final crystallographic R values are 21.3 and 19.4% for the two structures, respectively. In the Met121Ala mutant, the distance between the copper ion and His117 increases by 0.34 A compared with the wild-type structure. The removal of the methionine in the apical position induces a shortening of the distance from the copper ion to the carbonyl O atom of Gly45 from 2.97 to 2.74 A. In the Met121Ala-azide structure, the azide anion occupies the cavity created by replacing the Met121 side chain with the smaller methyl group of Ala. The azide anion binds with a terminal N atom to the copper ion at a distance of about 2.04 A. In addition, the copper ion has moved out of the trigonal plane by about 0.26 A towards the azide anion. Thus, the copper site in this structure has a distorted tetrahedral arrangement. The spectroscopic characteristics show, in addition, that the copper sites in the two structures are distinctively different. The Met121Ala mutant still maintains the properties of an ordinary type 1 copper site while the Met121Ala-azide derivative has an absorption maximum at about 409 nm and the copper hyperfine coupling has increased to a value intermediate between those of type 2 copper and the wild-type azurin.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15299604}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15299604 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15299604}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Vanngard, T.]]
[[Category: Vanngard, T.]]
[[Category: Electron transport]]
[[Category: Electron transport]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:27:04 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:50:21 2008''

Revision as of 18:50, 27 July 2008

Image:2tsb.png

Template:STRUCTURE 2tsb

AZURIN MUTANT M121A-AZIDE

Template:ABSTRACT PUBMED 15299604

About this Structure

2TSB is a Single protein structure of sequence from Pseudomonas aeruginosa. This structure supersedes the now removed PDB entry 1tsb. Full crystallographic information is available from OCA.

Reference

Mutant Met121Ala of Pseudomonas aeruginosa azurin and its azide derivative: crystal structures and spectral properties., Tsai LC, Bonander N, Harata K, Karlsson G, Vanngard T, Langer V, Sjolin L, Acta Crystallogr D Biol Crystallogr. 1996 Sep 1;52(Pt 5):950-8. PMID:15299604

Page seeded by OCA on Sun Jul 27 21:50:21 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2tsb"
Personal tools