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2jat

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[[Image:2jat.gif|left|200px]]
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{{STRUCTURE_2jat| PDB=2jat | SCENE= }}
{{STRUCTURE_2jat| PDB=2jat | SCENE= }}
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'''STRUCTURE OF DEOXYADENOSINE KINASE FROM M.MYCOIDES WITH PRODUCTS DCMP AND A FLEXIBLE DCDP BOUND'''
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===STRUCTURE OF DEOXYADENOSINE KINASE FROM M.MYCOIDES WITH PRODUCTS DCMP AND A FLEXIBLE DCDP BOUND===
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==Overview==
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Deoxyribonucleoside kinases (dNKs) catalyze the transfer of a phosphoryl group from ATP to a deoxyribonucleoside (dN), a key step in DNA precursor synthesis. Recently structural information concerning dNKs has been obtained, but no structure of a bacterial dCK/dGK enzyme is known. Here we report the structure of such an enzyme, represented by deoxyadenosine kinase from Mycoplasma mycoides subsp. mycoides small colony type (Mm-dAK). Superposition of Mm-dAK with its human counterpart's deoxyguanosine kinase (dGK) and deoxycytidine kinase (dCK) reveals that the overall structures are very similar with a few amino acid alterations in the proximity of the active site. To investigate the substrate specificity, Mm-dAK has been crystallized in complex with dATP and dCTP, as well as the products dCMP and dCDP. Both dATP and dCTP bind to the enzyme in a feedback-inhibitory manner with the dN part in the deoxyribonucleoside binding site and the triphosphates in the P-loop. Substrate specificity studies with clinically important nucleoside analogs as well as several phosphate donors were performed. Thus, in this study we combine structural and kinetic data to gain a better understanding of the substrate specificity of the dCK/dGK family of enzymes. The structure of Mm-dAK provides a starting point for making new anti bacterial agents against pathogenic bacteria.
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{{ABSTRACT_PUBMED_17229440}}
==About this Structure==
==About this Structure==
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[[Category: Kinase]]
[[Category: Kinase]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:51:02 2008''

Revision as of 18:51, 27 July 2008

Image:2jat.png

Template:STRUCTURE 2jat

STRUCTURE OF DEOXYADENOSINE KINASE FROM M.MYCOIDES WITH PRODUCTS DCMP AND A FLEXIBLE DCDP BOUND

Template:ABSTRACT PUBMED 17229440

About this Structure

2JAT is a Single protein structure of sequence from Mycoplasma mycoides subsp. mycoides sc. Full crystallographic information is available from OCA.

Reference

Structure-function analysis of a bacterial deoxyadenosine kinase reveals the basis for substrate specificity., Welin M, Wang L, Eriksson S, Eklund H, J Mol Biol. 2007 Mar 9;366(5):1615-23. Epub 2006 Dec 8. PMID:17229440

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