4rnp
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(New page: 200px<br /><applet load="4rnp" size="450" color="white" frame="true" align="right" spinBox="true" caption="4rnp, resolution 3.0Å" /> '''BACTERIOPHAGE T7 RNA ...)
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Revision as of 17:40, 20 November 2007
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BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY
Overview
The crystal structure of T7 RNA polymerase reveals a molecule organized, around a cleft that can accommodate a double-stranded DNA template. A, portion (approximately 45%) of the molecule displays extensive structural, homology to the polymerase domain of Klenow fragment and more limited, homology to the human immunodeficiency virus HIV-1 reverse transcriptase., A comparison of the structures and sequences of these polymerases, identifies structural elements that may be responsible for discriminating, between ribonucleotide and deoxyribonucleotide substrates, and RNA and DNA, templates. The relative locations of the catalytic site and a specific, promoter recognition residue allow the orientation of the polymerase on, the template to be defined.
About this Structure
4RNP is a Single protein structure of sequence from Bacteriophage t7. This structure superseeds the now removed PDB entries 3RNP and 2RNP. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Crystal structure of bacteriophage T7 RNA polymerase at 3.3 A resolution., Sousa R, Chung YJ, Rose JP, Wang BC, Nature. 1993 Aug 12;364(6438):593-9. PMID:7688864
Page seeded by OCA on Tue Nov 20 19:47:11 2007
