3hts
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(New page: 200px<br /><applet load="3hts" size="450" color="white" frame="true" align="right" spinBox="true" caption="3hts, resolution 1.75Å" /> '''HEAT SHOCK TRANSCRIP...)
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Revision as of 17:40, 20 November 2007
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HEAT SHOCK TRANSCRIPTION FACTOR/DNA COMPLEX
Overview
The 1.75 A crystal structure of the Kluyveromyces lactis heat shock, transcription factor (HSF) DNA-binding domain (DBD) complexed with DNA, reveals a protein-DNA interface with few direct major groove contacts and, a number of phosphate backbone contacts that are primarily water-mediated, interactions. The DBD, a 'winged' helix-turn-helix protein, displays a, novel mode of binding in that the 'wing' does not contact DNA like all, others of that class. Instead, the monomeric DBD, which crystallized as a, symmetric dimer to a pair of nGAAn inverted repeats, uses the 'wing' to, form part of the protein-protein contacts. This dimer interface is likely, important for increasing the DNA-binding specificity and affinity of the, trimeric form of HSF, as well as for increasing cooperativity between, adjacent trimers.
About this Structure
3HTS is a Single protein structure of sequence from Kluyveromyces lactis with GOL as ligand. Full crystallographic information is available from OCA.
Reference
A new use for the 'wing' of the 'winged' helix-turn-helix motif in the HSF-DNA cocrystal., Littlefield O, Nelson HC, Nat Struct Biol. 1999 May;6(5):464-70. PMID:10331875
Page seeded by OCA on Tue Nov 20 19:47:28 2007
