4rub
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(New page: 200px<br /><applet load="4rub" size="450" color="white" frame="true" align="right" spinBox="true" caption="4rub, resolution 2.7Å" /> '''A CRYSTAL FORM OF RIB...)
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Revision as of 17:40, 20 November 2007
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A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE
Overview
A new crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC, 4.1.1.39) from Nicotiana tabacum has been obtained at alkaline pH with, polyethylene glycol 8000 in the presence of a non-ionic detergent, beta-octyl glucoside. The crystals are grown at room temperature by the, hanging-drop vapor diffusion technique from a protein solution containing, enzyme complexed with CO2, Mg2+, and the transition state analog, 2-C-carboxy-D-arabinitol-1,5-bisphosphate. The crystals belong to the the, space group P3(1)21 (or P3(2)21) with the cell parameters a = 204.6 A, and, c = 117.4 A (1 A = 0.1 nm). The asymmetric unit contains half (L4S4: L, large subunit, 53,000 Mr; S, small subunit, 15,000 Mr) of a hexadecameric, molecule (L8S8, 540,000 Mr). The crystals diffract to at least 2.6 A Bragg, spacing and are suitable for X-ray structure determination.
About this Structure
4RUB is a Protein complex structure of sequences from Nicotiana tabacum with MG, CAP and FMT as ligands. Active as Ribulose-bisphosphate carboxylase, with EC number 4.1.1.39 Full crystallographic information is available from OCA.
Reference
A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state., Suh SW, Cascio D, Chapman MS, Eisenberg D, J Mol Biol. 1987 Sep 20;197(2):363-5. PMID:3681999
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