2r0g

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2r0g.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2r0g.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2r0g| PDB=2r0g | SCENE= }}
{{STRUCTURE_2r0g| PDB=2r0g | SCENE= }}
-
'''Chromopyrrolic acid-soaked RebC with bound 7-carboxy-K252c'''
+
===Chromopyrrolic acid-soaked RebC with bound 7-carboxy-K252c===
-
==Overview==
+
<!--
-
The biosynthesis of rebeccamycin, an antitumor compound, involves the remarkable eight-electron oxidation of chlorinated chromopyrrolic acid. Although one rebeccamycin biosynthetic enzyme is capable of generating low levels of the eight-electron oxidation product on its own, a second protein, RebC, is required to accelerate product formation and eliminate side reactions. However, the mode of action of RebC was largely unknown. Using crystallography, we have determined a likely function for RebC as a flavin hydroxylase, captured two snapshots of its dynamic catalytic cycle, and trapped a reactive molecule, a putative substrate, in its binding pocket. These studies strongly suggest that the role of RebC is to sequester a reactive intermediate produced by its partner protein and to react with it enzymatically, preventing its conversion to a suite of degradation products that includes, at low levels, the desired product.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17873060}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17873060 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17873060}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Monooxygenase]]
[[Category: Monooxygenase]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:01:20 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:13:50 2008''

Revision as of 19:14, 27 July 2008

Image:2r0g.png

Template:STRUCTURE 2r0g

Chromopyrrolic acid-soaked RebC with bound 7-carboxy-K252c

Template:ABSTRACT PUBMED 17873060

About this Structure

2R0G is a Single protein structure of sequence from Lechevalieria aerocolonigenes. Full crystallographic information is available from OCA.

Reference

Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC., Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan CL, Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15311-6. Epub 2007 Sep 14. PMID:17873060

Page seeded by OCA on Sun Jul 27 22:13:50 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2r0g"
Personal tools