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| - | [[Image:1rgv.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1rgv| PDB=1rgv | SCENE= }} | | {{STRUCTURE_1rgv| PDB=1rgv | SCENE= }} |
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| - | '''Crystal Structure of the Ferredoxin from Thauera aromatica'''
| + | ===Crystal Structure of the Ferredoxin from Thauera aromatica=== |
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| - | ==Overview==
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| - | 4-Hydroxybenzoyl-CoA reductase (4-HBCR) is a central enzyme in the metabolism of phenolic compounds in anaerobic bacteria. The enzyme catalyzes the reductive removal of the phenolic hydroxyl group from 4-hydroxybenzoyl-CoA, yielding benzoyl-CoA and water. 4-HBCR belongs to the xanthine oxidase (XO) family of molybdenum enzymes which occur as heterodimers, (alphabetagamma)(2). 4-HBCR contains two molybdopterins, four [2Fe-2S] and two [4Fe-4S] clusters and two FADs. A low-potential Allochromatium vinosum-type ferredoxin containing two [4Fe-4S] clusters serves as an in vivo electron donor for 4-HBCR. In this work, the oxygen-sensitive proteins 4-HBCR and the ferredoxin (TaFd) from Thauera aromatica were crystallized under anaerobic conditions. 4-HBCR crystallized with PEG 4000 and MPD as precipitant diffracted to about 1.6 A resolution and the crystals were highly suitable for X-ray structure analysis. Crystals of TaFd were obtained with (NH(4))(3)PO(4) as precipitant and revealed a solvent content of 77%, which is remarkably high for a small soluble protein. The structure of TaFd was solved at 2.9 A resolution by the molecular-replacement method using the highly related structure of the ferredoxin (CvFd) from A. vinosum as a model. Structural changes between the two ferredoxins around the [4Fe-4S] cluster can be correlated with their different redox potentials.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_14747735}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 14747735 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_14747735}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Warkentin, E.]] | | [[Category: Warkentin, E.]] |
| | [[Category: Electron transport]] | | [[Category: Electron transport]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:28:54 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:21:17 2008'' |
Revision as of 19:21, 27 July 2008
Template:STRUCTURE 1rgv
Crystal Structure of the Ferredoxin from Thauera aromatica
Template:ABSTRACT PUBMED 14747735
About this Structure
1RGV is a Single protein structure of sequence from Thauera aromatica. Full crystallographic information is available from OCA.
Reference
Crystallization of 4-hydroxybenzoyl-CoA reductase and the structure of its electron donor ferredoxin., Unciuleac M, Boll M, Warkentin E, Ermler U, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):388-91. Epub 2004, Jan 23. PMID:14747735
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