1tvb

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[[Image:1tvb.gif|left|200px]]
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{{STRUCTURE_1tvb| PDB=1tvb | SCENE= }}
{{STRUCTURE_1tvb| PDB=1tvb | SCENE= }}
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'''Crystal structure of Melanoma Antigen gp100(209-217) Bound to Human Class I MHC HLA-A2'''
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===Crystal structure of Melanoma Antigen gp100(209-217) Bound to Human Class I MHC HLA-A2===
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==Overview==
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The use of "anchor-fixed" altered peptide ligands is of considerable interest in the development of therapeutic vaccines for cancer and infectious diseases, but the mechanism by which successful altered peptide ligands elicit enhanced immunity is unclear. In this study, we have determined the crystallographic structure of a major tumor rejection Ag, gp100(209-217), in complex with the HLA-A*0201 (HLA-A2) molecule, as well as the structure of a modified version of the peptide which substitutes methionine for threonine at position 2 (T2M; gp100(209-2M)). The T2M-modified peptide, which is more immunogenic in vitro and in vivo, binds HLA-A2 with a approximately 9-fold greater affinity and has a approximately 7-fold slower dissociation rate at physiological temperature. Within the limit of the crystallographic data, the T2M substitution does not alter the structure of the peptide/HLA-A2 complex. Consistent with this finding, in peripheral blood from 95 human subjects, we were unable to identify higher frequencies of T cells specific for either the native or modified peptide. These data strongly support the conclusion that the greater immunogenicity of the gp100(209-2M) peptide is due to the enhanced stability of the peptide/MHC complex, validating the anchor-fixing approach for generating therapeutic vaccine candidates. Thermodynamic data suggest that the enhanced stability of the T2M-modified peptide/HLA-A2 complex is attributable to the increased hydrophobicity of the modified peptide, but the gain due to hydrophobicity is offset considerably by the loss of a hydrogen bond made by the native peptide to the HLA-A2 molecule. Our findings have broad implications for the optimization of current vaccine-design strategies.
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(as it appears on PubMed at http://www.pubmed.gov), where 15814707 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15814707}}
==Disease==
==Disease==
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[[Category: Peptide]]
[[Category: Peptide]]
[[Category: X-ray]]
[[Category: X-ray]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:24:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:24:11 2008''

Revision as of 19:24, 27 July 2008

Image:1tvb.png

Template:STRUCTURE 1tvb

Contents

Crystal structure of Melanoma Antigen gp100(209-217) Bound to Human Class I MHC HLA-A2

Template:ABSTRACT PUBMED 15814707

Disease

Known disease associated with this structure: Hypoproteinemia, hypercatabolic OMIM:[109700]

About this Structure

1TVB is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Increased immunogenicity of an anchor-modified tumor-associated antigen is due to the enhanced stability of the peptide/MHC complex: implications for vaccine design., Borbulevych OY, Baxter TK, Yu Z, Restifo NP, Baker BM, J Immunol. 2005 Apr 15;174(8):4812-20. PMID:15814707

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