1ovu

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{{STRUCTURE_1ovu| PDB=1ovu | SCENE= }}
{{STRUCTURE_1ovu| PDB=1ovu | SCENE= }}
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'''CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form I)'''
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===CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form I)===
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==Overview==
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Diffraction data from two non-isomorphous crystals (forms 1 and 2) of an artificial protein with a four-helix bundle motif, di-Co(II)-DF1-L13A, have been collected using synchrotron radiation. The phase of form 1 has been assigned using the group and minimal non-isomorphic supergroup relation between the space group of the previously determined di-Mn(II)-DF1-L13G structure and the space group of this form. This unconventional method of solving the phase problem has also been tested with form 2 using a reverse relation. The structure of the latter form has been solved using the group and maximal non-isomorphic subgroup relation with the space group of form 2 of the analogous dimanganese protein. This application has shown that this phasing method can be used for solving the protein structures of polymorphic crystals as an alternative to the molecular-replacement method.
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(as it appears on PubMed at http://www.pubmed.gov), where 12876346 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12876346}}
==About this Structure==
==About this Structure==
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[[Category: Alpha-helical bundle]]
[[Category: Alpha-helical bundle]]
[[Category: Protein design]]
[[Category: Protein design]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:20:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:15:06 2008''

Revision as of 20:15, 27 July 2008

Image:1ovu.png

Template:STRUCTURE 1ovu

CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form I)

Template:ABSTRACT PUBMED 12876346

About this Structure

Full crystallographic information is available from OCA.

Reference

Phasing protein structures using the group-subgroup relation., Di Costanzo L, Forneris F, Geremia S, Randaccio L, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1435-9. Epub 2003, Jul 23. PMID:12876346

Page seeded by OCA on Sun Jul 27 23:15:06 2008

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