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2dwr

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{{STRUCTURE_2dwr| PDB=2dwr | SCENE= }}
{{STRUCTURE_2dwr| PDB=2dwr | SCENE= }}
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'''Crystal structure of the human Wa rotavirus VP8* carbohydrate-recognising domain'''
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===Crystal structure of the human Wa rotavirus VP8* carbohydrate-recognising domain===
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==Overview==
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Rotavirus infection leads to the death of half a million children annually. The exact specifics of interaction between rotavirus particles and host cells enabling invasion and infection have remained elusive. Host cell oligosaccharides are critical components, and their involvement aids the virus in cell-recognition and attachment, as well as dictation of the remarkable host-specificity that rotaviruses demonstrate. Interaction between the rotavirus spike-protein carbohydrate-binding domain (VP8*) and cell surface oligosaccharides facilitate virus recognition of host cells and attachment. Rotaviruses are considered, controversially, to recognise vastly different carbohydrate structures and either with incorporation of terminal sialic acid or without, as assessed by their ability to infect cells that have been pre-treated with sialidases. Herein, the X-ray crystallographic structures of VP8* from the sialidase insensitive Wa and the sialidase sensitive CRW-8 rotavirus strains that cause debilitating gastroenteritis in human and pig are reported. Striking differences are apparent regarding recognition of the sialic acid derivative methyl alpha-D-N-acetylneuraminide, presenting the first experimental evidence of the inability of the human rotavirus strain to bind this monosaccharide, that correlates with Wa and CRW-8 recognising sialidase-resistant and sialidase-sensitive receptors, respectively. Identified are structural features that provide insight in attainment of substrate specificity exhibited by porcine strains as compared to rhesus rotavirus. Revealed in the CRW-8 VP8* structure is an additional bound ligand that intriguingly, is within a cleft located equivalent to the carbohydrate-binding region of galectins, and is suggestive of a new region for interaction with cell-surface carbohydrates. This novel result and detailed comparison of our representative sialidase-sensitive CRW-8 and insensitive Wa VP8* structures with those reported leads to our hypothesis that this groove is used for binding carbohydrates, and that for the human strains, as for other sialidase-insensitive strains could represent a major oligosaccharide-binding region.
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(as it appears on PubMed at http://www.pubmed.gov), where 17306299 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17306299}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Insight into host cell carbohydrate-recognition by human and porcine rotavirus from crystal structures of the virion spike associated carbohydrate-binding domain (VP8*)., Blanchard H, Yu X, Coulson BS, von Itzstein M, J Mol Biol. 2007 Apr 6;367(4):1215-26. Epub 2007 Jan 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17306299 17306299]
Insight into host cell carbohydrate-recognition by human and porcine rotavirus from crystal structures of the virion spike associated carbohydrate-binding domain (VP8*)., Blanchard H, Yu X, Coulson BS, von Itzstein M, J Mol Biol. 2007 Apr 6;367(4):1215-26. Epub 2007 Jan 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17306299 17306299]
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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the VP8* carbohydrate-binding protein of the human rotavirus strain Wa., Kraschnefski MJ, Scott SA, Holloway G, Coulson BS, von Itzstein M, Blanchard H, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Nov 1;61(Pt, 11):989-93. Epub 2005 Oct 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511215 16511215]
[[Category: Human rotavirus a]]
[[Category: Human rotavirus a]]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Blanchard, H.]]
[[Category: Blanchard, H.]]
[[Category: Beta-sandwich]]
[[Category: Beta-sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:32:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:16:00 2008''

Revision as of 20:16, 27 July 2008

Image:2dwr.png

Template:STRUCTURE 2dwr

Crystal structure of the human Wa rotavirus VP8* carbohydrate-recognising domain

Template:ABSTRACT PUBMED 17306299

About this Structure

2DWR is a Single protein structure of sequence from Human rotavirus a. Full crystallographic information is available from OCA.

Reference

Insight into host cell carbohydrate-recognition by human and porcine rotavirus from crystal structures of the virion spike associated carbohydrate-binding domain (VP8*)., Blanchard H, Yu X, Coulson BS, von Itzstein M, J Mol Biol. 2007 Apr 6;367(4):1215-26. Epub 2007 Jan 13. PMID:17306299

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the VP8* carbohydrate-binding protein of the human rotavirus strain Wa., Kraschnefski MJ, Scott SA, Holloway G, Coulson BS, von Itzstein M, Blanchard H, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Nov 1;61(Pt, 11):989-93. Epub 2005 Oct 20. PMID:16511215

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