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1xti

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[[Image:1xti.gif|left|200px]]
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{{STRUCTURE_1xti| PDB=1xti | SCENE= }}
{{STRUCTURE_1xti| PDB=1xti | SCENE= }}
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'''Structure of Wildtype human UAP56'''
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===Structure of Wildtype human UAP56===
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==Overview==
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Pre-mRNA splicing requires the function of a number of RNA-dependent ATPases/helicases, yet no three-dimensional structure of any spliceosomal ATPases/helicases is known. The highly conserved DECD-box protein UAP56/Sub2 is an essential splicing factor that is also important for mRNA export. The expected ATPase/helicase activity appears to be essential for the UAP56/Sub2 functions. Here, we show that purified human UAP56 is an active RNA-dependent ATPase, and we also report the crystal structures of UAP56 alone and in complex with ADP, as well as a DECD to DEAD mutant. The structures reveal a unique spatial arrangement of the two conserved helicase domains, and ADP-binding induces significant conformational changes of key residues in the ATP-binding pocket. Our structural analyses suggest a specific protein-RNA displacement model of UAP56/Sub2. The detailed structural information provides important mechanistic insights into the splicing function of UAP56/Sub2. The structures also will be useful for the analysis of other spliceosomal DExD-box ATPases/helicases.
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The line below this paragraph, {{ABSTRACT_PUBMED_15585580}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15585580 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15585580}}
==About this Structure==
==About this Structure==
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[[Category: Xu, R M.]]
[[Category: Xu, R M.]]
[[Category: Alpha-beta fold]]
[[Category: Alpha-beta fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:29:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:17:07 2008''

Revision as of 20:17, 27 July 2008

Image:1xti.png

Template:STRUCTURE 1xti

Structure of Wildtype human UAP56

Template:ABSTRACT PUBMED 15585580

About this Structure

1XTI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of the human ATP-dependent splicing and export factor UAP56., Shi H, Cordin O, Minder CM, Linder P, Xu RM, Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17628-33. Epub 2004 Dec 7. PMID:15585580

Page seeded by OCA on Sun Jul 27 23:17:07 2008

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