1kuj
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(New page: 200px<br /><applet load="1kuj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kuj, resolution 2.00Å" /> '''Crystal structure of...)
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Revision as of 17:46, 20 November 2007
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Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose
Overview
Evidence is presented that the specificity of jacalin, the seed lectin, from jack fruit (Artocarpus integrifolia), is not directed exclusively, against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and, galactose, but also against mannose and oligomannosides. Biochemical, analyses based on surface-plasmon-resonance measurements, combined with, the X-ray-crystallographic determination of the structure of a, jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated, clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and, N-acetylmuramic acid. Structural analyses demonstrated that the relatively, large size of the carbohydrate-binding site enables jacalin to accommodate, monosaccharides with different hydroxyl conformations and provided, unambiguous evidence that the beta-prism structure of jacalin is a, sufficiently flexible structural scaffold to confer different, carbohydrate-binding specificities to a single lectin.
About this Structure
1KUJ is a Protein complex structure of sequences from Artocarpus integer with MMA as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose., Bourne Y, Astoul CH, Zamboni V, Peumans WJ, Menu-Bouaouiche L, Van Damme EJ, Barre A, Rouge P, Biochem J. 2002 May 15;364(Pt 1):173-80. PMID:11988090
Page seeded by OCA on Tue Nov 20 19:53:20 2007
