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1x3e

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{{STRUCTURE_1x3e| PDB=1x3e | SCENE= }}
{{STRUCTURE_1x3e| PDB=1x3e | SCENE= }}
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'''Crystal structure of the single-stranded DNA-binding protein from Mycobacterium smegmatis'''
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===Crystal structure of the single-stranded DNA-binding protein from Mycobacterium smegmatis===
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==Overview==
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The structure of Mycobacterium smegmatis single-stranded DNA-binding protein (SSB) has been determined using three data sets collected from related crystals. The structure is similar to that of its homologue from Mycobacterium tuberculosis, indicating that the clamp arrangement that stabilizes the dimer and the ellipsoidal shape of the tetramer are characteristic features of mycobacterial SSBs. The central OB fold is conserved in mycobacterial SSBs as well as those from Escherichia coli, Deinococcus radiodurans and human mitochondria. However, the quaternary structure exhibits considerable variability. The observed plasticity of the subunit is related to this variability. The crystal structures and modelling provide a rationale for the variability. The strand involved in the clamp mechanism, which leads to higher stability of the tetramer, appears to occur in all high-G+C Gram-positive bacteria. The higher stability is perhaps required by these organisms. The mode of DNA binding of mycobacterial SSBs is different from that of E. coli SSB partly on account of the difference in the shape of the tetramers. Another difference between the two modes is that the former contains additional ionic interactions and is more susceptible to salt concentration.
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The line below this paragraph, {{ABSTRACT_PUBMED_16041080}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16041080 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16041080}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structure of Mycobacterium smegmatis single-stranded DNA-binding protein and a comparative study involving homologus SSBs: biological implications of structural plasticity and variability in quaternary association., Saikrishnan K, Manjunath GP, Singh P, Jeyakanthan J, Dauter Z, Sekar K, Muniyappa K, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1140-8. Epub 2005, Jul 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16041080 16041080]
Structure of Mycobacterium smegmatis single-stranded DNA-binding protein and a comparative study involving homologus SSBs: biological implications of structural plasticity and variability in quaternary association., Saikrishnan K, Manjunath GP, Singh P, Jeyakanthan J, Dauter Z, Sekar K, Muniyappa K, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1140-8. Epub 2005, Jul 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16041080 16041080]
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Structure of Mycobacterium tuberculosis single-stranded DNA-binding protein. Variability in quaternary structure and its implications., Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Sekar K, Varshney U, Vijayan M, J Mol Biol. 2003 Aug 8;331(2):385-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12888346 12888346]
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Crystallization and preliminary X-ray studies of the single-stranded DNA-binding protein from Mycobacterium tuberculosis., Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Purnapatre K, Sekar K, Varshney U, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2002 Feb;58(Pt 2):327-9. Epub 2002, Jan 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11807266 11807266]
[[Category: Mycobacterium smegmatis]]
[[Category: Mycobacterium smegmatis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Dna-binding protein]]
[[Category: Dna-binding protein]]
[[Category: Oligonucleotide binding fold]]
[[Category: Oligonucleotide binding fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:28:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:22:16 2008''

Revision as of 20:22, 27 July 2008

Image:1x3e.png

Template:STRUCTURE 1x3e

Crystal structure of the single-stranded DNA-binding protein from Mycobacterium smegmatis

Template:ABSTRACT PUBMED 16041080

About this Structure

1X3E is a Single protein structure of sequence from Mycobacterium smegmatis. Full crystallographic information is available from OCA.

Reference

Structure of Mycobacterium smegmatis single-stranded DNA-binding protein and a comparative study involving homologus SSBs: biological implications of structural plasticity and variability in quaternary association., Saikrishnan K, Manjunath GP, Singh P, Jeyakanthan J, Dauter Z, Sekar K, Muniyappa K, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1140-8. Epub 2005, Jul 20. PMID:16041080

Structure of Mycobacterium tuberculosis single-stranded DNA-binding protein. Variability in quaternary structure and its implications., Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Sekar K, Varshney U, Vijayan M, J Mol Biol. 2003 Aug 8;331(2):385-93. PMID:12888346

Crystallization and preliminary X-ray studies of the single-stranded DNA-binding protein from Mycobacterium tuberculosis., Saikrishnan K, Jeyakanthan J, Venkatesh J, Acharya N, Purnapatre K, Sekar K, Varshney U, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2002 Feb;58(Pt 2):327-9. Epub 2002, Jan 24. PMID:11807266

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