2c77

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2c77.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2c77.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2c77| PDB=2c77 | SCENE= }}
{{STRUCTURE_2c77| PDB=2c77 | SCENE= }}
-
'''EF-TU COMPLEXED WITH A GTP ANALOG AND THE ANTIBIOTIC GE2270 A'''
+
===EF-TU COMPLEXED WITH A GTP ANALOG AND THE ANTIBIOTIC GE2270 A===
-
==Overview==
+
<!--
-
Pulvomycin inhibits protein synthesis by preventing the formation of the ternary complex between elongation factor Tu (EF-Tu) x GTP and aa-tRNA. In this work, the crystal structure of Thermus thermophilus EF-Tu x pulvomycin in complex with the GTP analogue guanylyl imino diphosphate (GDPNP) at 1.4 A resolution reveals an antibiotic binding site extending from the domain 1-3 interface to domain 2, overlapping the domain 1-2-3 junction. Pulvomycin binding interferes with the binding of the 3'-aminoacyl group, the acceptor stem, and 5' end of tRNA. Only part of pulvomycin overlaps the binding site of GE2270 A, a domain 2-bound antibiotic of a structure unrelated to pulvomycin, which also hinders aa-tRNA binding. The structure of the T. thermophilus EF-Tu x GDPNP x GE2270 A complex at 1.6 A resolution shows that GE2270 A interferes with the binding of the 3'-aminoacyl group and part of the acceptor stem of aa-tRNA but not with the 5' end. Both compounds, pulvomycin more markedly, hinder the correct positioning of domain 1 over domains 2 and 3 that characterizes the active form of EF-Tu, while they affect the domain 1 switch regions that control the EF-Tu x GDP/GTP transitions in different ways. This work reveals how two antibiotics with different structures and binding modes can employ a similar mechanism of action.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16734421}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16734421 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16734421}}
==About this Structure==
==About this Structure==
Line 38: Line 42:
[[Category: Protein synthesis]]
[[Category: Protein synthesis]]
[[Category: Translation elongation factor]]
[[Category: Translation elongation factor]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:22:06 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:33:06 2008''

Revision as of 20:33, 27 July 2008

Image:2c77.png

Template:STRUCTURE 2c77

EF-TU COMPLEXED WITH A GTP ANALOG AND THE ANTIBIOTIC GE2270 A

Template:ABSTRACT PUBMED 16734421

About this Structure

2C77 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Structural basis of the action of pulvomycin and GE2270 A on elongation factor Tu., Parmeggiani A, Krab IM, Okamura S, Nielsen RC, Nyborg J, Nissen P, Biochemistry. 2006 Jun 6;45(22):6846-57. PMID:16734421

Page seeded by OCA on Sun Jul 27 23:33:06 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2c77"
Personal tools