1kvn
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(New page: 200px<br /><applet load="1kvn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kvn" /> '''Solution Structure Of Protein SRP19 Of The A...)
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Revision as of 17:48, 20 November 2007
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Solution Structure Of Protein SRP19 Of The Arhaeoglobus fulgidus Signal Recognition Particle, 10 Structures
Overview
Protein SRP19 is an essential RNA-binding component of the signal, recognition particle (SRP) in Archaea and Eucarya. A three-dimensional, solution structure of the 104 residue SRP19 from the hyperthermophilic, archaeon Archaeoglobus fulgidus, designated as Af19, was determined by NMR, spectroscopy. Af19 contains three beta-strands, two alpha-helical regions, arranged in a betaalphabetabetaalpha topology, a 3(10) helix, and a, disordered C-terminal tail. This fold is similar to the, betaalphabetabetaalphabeta RNP motif present in numerous other RNA-binding, proteins, which engage their cognate RNAs using conserved sequence motifs, present within beta-strands 1 and 3. Mutagenesis studies of human SRP19, however, reveal the major contact sites with SRP RNA reside within loops, 1, 3, and 4. These contacts were verified by the crystal structure of, human SRP19 complexed to SRP RNA helix 6 reported subsequent to the, submission of the manuscript. The crystal structure also reveals that, unlike canonical RNP motifs, SRP19 does not engage specific RNA bases, through conserved sequence motifs present within beta-strands 1 and 3., Instead, SRP19 uses residues both within and flanking beta-strand 1 to, stabilize the complex through direct and indirect contacts to the, phosphate backbone of the tetraloop, leaving the bases of the tetraloop, exposed. This, coupled with the fact that SRP19 appears relatively rigid, and undergoes only minor changes in structure upon RNA binding, may, underlie the molecular basis by which SRP19 functions to initiate SRP, assembly.
About this Structure
1KVN is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
Solution structure of protein SRP19 of Archaeoglobus fulgidus signal recognition particle., Pakhomova ON, Deep S, Huang Q, Zwieb C, Hinck AP, J Mol Biol. 2002 Mar 15;317(1):145-58. PMID:11916385
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