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1wpn

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{{STRUCTURE_1wpn| PDB=1wpn | SCENE= }}
{{STRUCTURE_1wpn| PDB=1wpn | SCENE= }}
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'''Crystal structure of the N-terminal core of Bacillus subtilis inorganic pyrophosphatase'''
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===Crystal structure of the N-terminal core of Bacillus subtilis inorganic pyrophosphatase===
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==Overview==
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Family II inorganic pyrophosphatases (PPases) constitute a new evolutionary group of PPases, with a different fold and mechanism than the common family I enzyme; they are related to the "DHH" family of phosphoesterases. Biochemical studies have shown that Mn(2+) and Co(2+) preferentially activate family II PPases; Mg(2+) partially activates; and Zn(2+) can either activate or inhibit (Zyryanov et al., Biochemistry, 43, 14395-14402, accompanying paper in this issue). The three solved family II PPase structures did not explain the differences between the PPase families nor the metal ion differences described above. We therefore solved three new family II PPase structures: Bacillus subtilis PPase (Bs-PPase) dimer core bound to Mn(2+) at 1.3 A resolution, and, at 2.05 A resolution, metal-free Bs-PPase and Streptococcus gordonii (Sg-PPase) containing sulfate and Zn(2+). Comparison of the new and old structures of various family II PPases demonstrates why the family II enzyme prefers Mn(2+) or Co(2+), as an activator rather than Mg(2+). Both M1 and M2 undergo significant changes upon substrate binding, changing from five-coordinate to octahedral geometry. Mn(2+) and Co(2+), which readily adopt different coordination states and geometries, are thus favored. Combining our structures with biochemical data, we identified M2 as the high-affinity metal site. Zn(2+) activates in the M1 site, where octahedral geometry is not essential for catalysis, but inhibits in the M2 site, because it is unable to assume octahedral geometry but remains trigonal bipyramidal. Finally, we propose that Lys205-Gln81-Gln80 form a hydrophilic channel to speed product release from the active site.
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==About this Structure==
==About this Structure==
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[[Category: Inorganic pyrophosphatase]]
[[Category: Inorganic pyrophosphatase]]
[[Category: Metal binding]]
[[Category: Metal binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:50:50 2008''

Revision as of 20:50, 27 July 2008

Image:1wpn.png

Template:STRUCTURE 1wpn

Crystal structure of the N-terminal core of Bacillus subtilis inorganic pyrophosphatase

Template:ABSTRACT PUBMED 15533045

About this Structure

1WPN is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structural studies of metal ions in family II pyrophosphatases: the requirement for a Janus ion., Fabrichniy IP, Lehtio L, Salminen A, Zyryanov AB, Baykov AA, Lahti R, Goldman A, Biochemistry. 2004 Nov 16;43(45):14403-11. PMID:15533045

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