1yvu

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[[Image:1yvu.gif|left|200px]]
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{{STRUCTURE_1yvu| PDB=1yvu | SCENE= }}
{{STRUCTURE_1yvu| PDB=1yvu | SCENE= }}
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'''Crystal structure of A. aeolicus Argonaute'''
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===Crystal structure of A. aeolicus Argonaute===
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==Overview==
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Argonaute (Ago) proteins constitute a key component of the RNA-induced silencing complex (RISC). We report the crystal structure of Aquifex aeolicus Ago (Aa-Ago) together with binding and cleavage studies, which establish this eubacterial Ago as a bona fide guide DNA strand-mediated site-specific RNA endonuclease. We have generated a stereochemically robust model of the complex, where the guide DNA-mRNA duplex is positioned within a basic channel spanning the bilobal interface, such that the 5' phosphate of the guide strand can be anchored in a basic pocket, and the mRNA can be positioned for site-specific cleavage by RNase H-type divalent cation-coordinated catalytic Asp residues of the PIWI domain. Domain swap experiments involving chimeras of human Ago (hAgo1) and cleavage-competent hAgo2 reinforce the role of the PIWI domain in "slicer" activity. We propose a four-step Ago-mediated catalytic cleavage cycle model, which provides distinct perspectives into the mechanism of guide strand-mediated mRNA cleavage within the RISC.
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The line below this paragraph, {{ABSTRACT_PUBMED_16061186}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16061186 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16061186}}
==About this Structure==
==About this Structure==
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[[Category: Zhadina, M.]]
[[Category: Zhadina, M.]]
[[Category: Rnase h fold]]
[[Category: Rnase h fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:51:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:51:35 2008''

Revision as of 20:51, 27 July 2008

Image:1yvu.png

Template:STRUCTURE 1yvu

Crystal structure of A. aeolicus Argonaute

Template:ABSTRACT PUBMED 16061186

About this Structure

1YVU is a Single protein structure of sequence from Aquifex aeolicus vf5. Full crystallographic information is available from OCA.

Reference

Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage., Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, Dauter Z, Tuschl T, Patel DJ, Mol Cell. 2005 Aug 5;19(3):405-19. PMID:16061186

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