1rh8

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{{STRUCTURE_1rh8| PDB=1rh8 | SCENE= }}
{{STRUCTURE_1rh8| PDB=1rh8 | SCENE= }}
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'''Three-dimensional structure of the calcium-free Piccolo C2A-domain'''
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===Three-dimensional structure of the calcium-free Piccolo C2A-domain===
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==Overview==
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C2 domains are widespread Ca2+-binding modules. The active zone protein Piccolo (also known as Aczonin) contains an unusual C2A domain that exhibits a low affinity for Ca2+, a Ca2+-induced conformational change and Ca2+-dependent dimerization. We show here that removal of a nine-residue sequence by alternative splicing increases the Ca2+ affinity, abolishes the conformational change and abrogates dimerization of the Piccolo C2A domain. The NMR structure of the Ca2+-free long variant provides a structural basis for these different properties of the two splice forms, showing that the nine-residue sequence forms a beta-strand otherwise occupied by a nonspliced sequence. Consequently, Ca2+-binding to the long Piccolo C2A domain requires a marked rearrangement of secondary structure that cannot occur for the short variant. These results reveal a novel mechanism of action of C2 domains and uncover a structural principle that may underlie the alteration of protein function by short alternatively spliced sequences.
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{{ABSTRACT_PUBMED_14718922}}
==About this Structure==
==About this Structure==
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1RH8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH8 OCA].
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1RH8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH8 OCA].
==Reference==
==Reference==
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[[Category: Sugita, S.]]
[[Category: Sugita, S.]]
[[Category: Beta-sandwich]]
[[Category: Beta-sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:29:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:01:16 2008''

Revision as of 21:01, 27 July 2008

Image:1rh8.png

Template:STRUCTURE 1rh8

Three-dimensional structure of the calcium-free Piccolo C2A-domain

Template:ABSTRACT PUBMED 14718922

About this Structure

1RH8 is a Single protein structure of sequence from Rattus norvegicus. Full experimental information is available from OCA.

Reference

A conformational switch in the Piccolo C2A domain regulated by alternative splicing., Garcia J, Gerber SH, Sugita S, Sudhof TC, Rizo J, Nat Struct Mol Biol. 2004 Jan;11(1):45-53. Epub 2003 Dec 29. PMID:14718922

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